Faruqui Nilofar, Kummrow Andreas, Fu Boqiang, Divieto Carla, Rojas Fabiola, Kisulu Florence, Cavalcante Janaina J V, Wang Jing, Campbell Jonathan, Martins Juliana L, Choi Jun-Hyuk, Sassi Maria Paola, Zucco Massimo, Vonsky Maxim, Vessillier Sandrine, Zou Shan, Fujii Shin-Ichiro, Ryadnov Maxim G
National Physical Laboratory, Teddington, United Kingdom.
Physikalisch Technische Bundesanstalt, Berlin, Germany.
Front Bioeng Biotechnol. 2020 Jan 14;7:456. doi: 10.3389/fbioe.2019.00456. eCollection 2019.
The symptomatic irreproducibility of data in biomedicine and biotechnology prompts the need for higher order measurements of cells in their native and near-native environments. Such measurements may support the adoption of new technologies as well as the development of research programs across different sectors including healthcare and clinic, environmental control and national security. With an increasing demand for reliable cell-based products and services, cellular metrology is poised to help address current and emerging measurement challenges faced by end-users. However, metrological foundations in cell analysis remain sparse and significant advances are necessary to keep pace with the needs of modern medicine and industry. Herein we discuss a role of metrology in cell and cell-related R&D activities to underpin growing international measurement capabilities. Relevant measurands are outlined and the lack of reference methods and materials, particularly those based on functional cell responses in native environments, is highlighted. The status quo and current challenges in cellular measurements are discussed in the light of metrological traceability in cell analysis and applications (e.g., a functional cell count). An emphasis is made on the consistency of measurement results independent of the analytical platform used, high confidence in data quality vs. quantity, scale of measurements and issues of building infrastructure for end-users.
生物医学和生物技术中数据的症状不可重复性促使人们需要在细胞的天然和接近天然环境中进行更高阶的细胞测量。此类测量可能有助于采用新技术，并推动包括医疗保健和临床、环境控制和国家安全在内的不同领域研究项目的发展。随着对可靠的细胞基产品和服务的需求不断增加，细胞计量学有望帮助应对终端用户当前面临的以及新出现的测量挑战。然而，细胞分析中的计量基础仍然薄弱，需要取得重大进展才能跟上现代医学和工业的需求。在此，我们讨论计量学在细胞及与细胞相关的研发活动中的作用，以支撑不断增强的国际测量能力。概述了相关的被测量，并强调了缺乏参考方法和材料的问题，特别是那些基于细胞在天然环境中的功能反应的方法和材料。根据细胞分析和应用（如功能性细胞计数）中的计量溯源性，讨论了细胞测量的现状和当前面临的挑战。重点强调了测量结果的一致性，而与所使用的分析平台无关，对数据质量而非数量的高度信心，测量规模以及为终端用户建立基础设施的问题。
