School of Food Science and Technology, Jiangnan University, Wuxi, PR China.
Changzhou Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Changzhou, Jiangsu Province, China and Institute of Nutrition and Health, Qingdao University, Qingdao, PR China.
Food Funct. 2020 Feb 26;11(2):1513-1524. doi: 10.1039/c9fo02919b.
Gut microbiota play a crucial role in metabolic dysfunction during gestation, which might be prevented by using probiotics. This study compared the composition of the gut microbiota in healthy and complicated pregnancies, for screening and isolating healthy pregnancy-derived probiotics. According to the principal component analysis of secretory immunoglobulin A (SIgA)-coated microbiota in the gut, third-trimester volunteers can be divided into three groups: AHd (n = 29), GDMd (n = 37), and GHd (n = 25), dominated by asymptomatic healthy donors (62.07%), gestational diabetes mellitus (GDM) donors (40.54%), and gestational hypertension (GH) donors (40%), respectively. There was a significant difference in β-diversity (p < 0.01) and α-diversity (p < 0.05) among the three groups. At the phylum level, the Firmicutes of the GHd group were significantly lower than those of the AHd group (p = 0.039), while Bacteroidetes (p = 0.005) and Proteobacteria (p = 0.002) of the GHd group were more dominant than those of the AHd group. At the genus level, the linear discriminant analysis effect size showed that SIgA-targeted Enterococcus was the dominant taxonomic biomarker of the AHd group, and the GHd group was enriched with Escherichia and Streptococcus. The GDMd and GHd groups had higher faecal calprotectin, serum lipopolysaccharide, zonulin, and GLYCAM-1 levels. We conclude that the occurrence of complications in the third trimester may be related to intestinal barrier injury associated with disorders of the intestinal SIgA-targeted microbiota; gut barrier injury triggers inflammation in pregnant women. SIgA-targeted L. reuteri showed a significant correlation with low inflammatory response and may be a potential probiotic candidate for preventing pregnancy complications.
肠道微生物群在妊娠期间的代谢功能障碍中发挥着关键作用，使用益生菌可能预防这种情况。本研究比较了健康和复杂妊娠中肠道微生物群的组成，以筛选和分离健康妊娠衍生的益生菌。根据肠道分泌型免疫球蛋白 A（SIgA）包被菌群的主成分分析，可将孕晚期志愿者分为三组：AHd（n = 29）、GDMd（n = 37）和 GHd（n = 25），分别以无症状健康供体（62.07%）、妊娠糖尿病（GDM）供体（40.54%）和妊娠高血压（GH）供体（40%）为主。三组间β多样性（p < 0.01）和α多样性（p < 0.05）存在显著差异。在门水平上，GHd 组的厚壁菌门显著低于 AHd 组（p = 0.039），而 Bacteroidetes（p = 0.005）和 Proteobacteria（p = 0.002）在 GHd 组中的丰度高于 AHd 组。在属水平上，SIgA 靶向肠球菌是 AHd 组的主要分类生物标志物，而 GHd 组中埃希氏菌和链球菌富集。GDMd 和 GHd 组粪便钙卫蛋白、血清脂多糖、肠紧密连接蛋白和 GLYCAM-1 水平较高。我们得出结论，孕晚期并发症的发生可能与肠道 SIgA 靶向菌群紊乱相关的肠道屏障损伤有关；肠道屏障损伤引发孕妇炎症。SIgA 靶向的 L. reuteri 与低炎症反应显著相关，可能是预防妊娠并发症的潜在益生菌候选物。
