State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2867-2872. doi: 10.1099/ijsem.0.004112. Epub 2020 Mar 24.
A novel pink-pigmented strain, designated 6HR-1, was isolated from tungsten mine tailings in Jiangxi Province, PR China. Cells were Gram-stain-negative, aerobic, non-spore-forming, rod-shaped and motile with a polar flagellum (monotrichous). It could not utilize methanol, methylamine, formaldehyde or formate as a sole carbon source. The methanol dehydrogenase gene was absent but the gene was present. Phylogenomic and 16S rRNA gene phylogenetic analyses clearly showed that strain 6HR-1 was affiliated to the genus and closely related to '' 17Sr1-28 (98.6 %), JCM 14648 (97.7 %), DSM 16961 (97.7 %) and KACC 19662 (97.4 %). The average nucleotide identity and digital DNA-DNA hybridization values between strain 6HR-1 and its closely related type species were 87.4-88.7 and 33.2-36.3 %, respectively. It had summed feature 8 (C ω7 and/or C ω6) as the major fatty acid and ubiquinone 10 as the predominant respiratory quinone. Polyphasic characterization supported that strain 6HR-1 represents a novel species of the genus , for which the name sp. nov. is proposed with the type strain 6HR-1 (=GDMCC 1.662=KCTC 42760).
一株新型的粉红色着色菌株，命名为 6HR-1，从中国江西省的钨矿尾矿中分离得到。细胞革兰氏染色阴性，需氧，不产芽孢，杆状，有极性鞭毛（单端生），能运动。不能利用甲醇、甲胺、甲醛或甲酸盐作为唯一碳源。甲醇脱氢酶基因缺失，但存在基因。系统发育基因组和 16S rRNA 基因系统发育分析清楚地表明，菌株 6HR-1属于属，与“17Sr1-28（98.6%）、JCM 14648（97.7%）、DSM 16961（97.7%）和 KACC 19662（97.4%）密切相关。菌株 6HR-1与其密切相关的模式种之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 87.4-88.7%和 33.2-36.3%。它以 summed feature 8（C ω7 和/或 C ω6）作为主要脂肪酸，以泛醌 10 作为主要呼吸醌。多相特征支持菌株 6HR-1代表属的一个新种，因此提议使用 6HR-1 型菌株（=GDMCC 1.662=KCTC 42760）命名为 sp. nov.。
