Engineering Research Center in Biomaterials, Sichuan University, Chengdu, 610064, China; National Engineering Research Center for Biomaterials, Chengdu, 610064, China; Sichuan Guojia Biomaterials Co., Ltd, Chengdu, 610064, China.
Panzhihua International Research Institute of Vanadium and Titanium, Panzhihua University, 617000, Panzhihua, Sichuan, China.
Colloids Surf B Biointerfaces. 2020 Apr;188:110783. doi: 10.1016/j.colsurfb.2020.110783. Epub 2020 Jan 16.
The failure of dental implants is usually caused by bacteria infection, poor bioactivity and biocompatibility. It is a common phenomenon clinically. Statherin, a salivary protein, plays a crucial role of mediator between materials and cells/bacteria. However, the conformation of statherin might be changed by the implants in vivo. In this study, we investigated the effects of statherin on the bioactivities, antibacterial abilities and biocompatibilities of the titanium metals and the reaction mechanism. We found that the conformation of statherin was mainly influenced by surface composition, surface structure, surface roughness, surface hydrophilia and Ti-OH groups of materials. Statherin could decrease the cell biocompatibility of the titanium metals including pure titanium (PT), anodic oxidation (AO), sandblasting and etching (SLA) and plasma spraying hydroxyapatite (HA) coating in HGF cell experiments, regulate the bio-mineralization ability of HA coating in SBF, and enhance the antibacterial properties of PT and HA coating. This study revealed that surface properties of materials could change the conformation of statherin, which influenced the bioactivities, antibacterial properties and biocompatibilities of the materials in return.
种植体的失败通常是由细菌感染、生物活性和生物相容性差引起的。这在临床上是很常见的现象。唾液蛋白唾液组蛋白在材料与细胞/细菌之间起着重要的介质作用。然而，组蛋白的构象可能会在体内被种植体改变。在这项研究中，我们研究了组蛋白对钛金属的生物活性、抗菌能力和生物相容性的影响及其反应机制。我们发现，组蛋白的构象主要受材料的表面组成、表面结构、表面粗糙度、表面亲水性和 Ti-OH 基团的影响。在 HGF 细胞实验中，组蛋白可降低钛金属（包括纯钛（PT）、阳极氧化（AO）、喷砂酸蚀（SLA）和等离子喷涂羟基磷灰石（HA）涂层）的细胞生物相容性，调节 SBF 中 HA 涂层的生物矿化能力，并增强 PT 和 HA 涂层的抗菌性能。本研究表明，材料的表面性质可以改变组蛋白的构象，从而影响材料的生物活性、抗菌性能和生物相容性。
