National Institute of Science and Technology in Plant-Pest Interactions, Bioagro, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-000, Brazil; Departament of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-000, Brazil.
National Institute of Science and Technology in Plant-Pest Interactions, Bioagro, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-000, Brazil.
Plant Sci. 2020 Mar;292:110410. doi: 10.1016/j.plantsci.2020.110410. Epub 2020 Jan 9.
The Geminiviridae family is one of the most successful and largest families of plant viruses that infect a large variety of important dicotyledonous and monocotyledonous crops and cause significant yield losses worldwide. This broad spectrum of host range is only possible because geminiviruses have evolved sophisticated strategies to overcome the arsenal of antiviral defenses in such diverse plant species. In addition, geminiviruses evolve rapidly through recombination and pseudo-recombination to naturally create a great diversity of virus species with divergent genome sequences giving the virus an advantage over the host recognition system. Therefore, it is not surprising that efficient molecular strategies to combat geminivirus infection under open field conditions have not been fully addressed. In this review, we present the anti-geminiviral arsenal of plant defenses, the evolved virulence strategies of geminiviruses to overcome these plant defenses and the most recent strategies that have been engineered for transgenic resistance. Although, the in vitro reactivation of suppressed natural defenses as well as the use of RNAi and CRISPR/Cas systems hold the potential for achieving broad-range resistance and/or immunity, potential drawbacks have been associated with each case.
双生病毒科是感染多种重要双子叶和单子叶作物的最成功和最大的植物病毒科之一，在全球范围内造成了重大的产量损失。这种广泛的宿主范围之所以成为可能，是因为双生病毒已经进化出了复杂的策略，可以克服如此多样化的植物物种中的抗病毒防御系统。此外，双生病毒通过重组和伪重组迅速进化，自然产生具有不同基因组序列的大量病毒物种，使病毒在与宿主识别系统的竞争中具有优势。因此，在野外条件下对抗双生病毒感染的有效分子策略尚未得到充分解决，这并不奇怪。在这篇综述中，我们介绍了植物防御的抗病毒武器库、双生病毒为克服这些植物防御而进化出的毒力策略，以及为转基因抗性而设计的最新策略。尽管抑制的天然防御的体外再激活以及 RNAi 和 CRISPR/Cas 系统的使用具有实现广谱抗性和/或免疫的潜力，但每种情况都存在潜在的缺点。
