Zhu Wenbin, Pirovano Giacomo, O'Neal Patrick K, Gong Cheng, Kulkarni Nachiket, Nguyen Christopher D, Brand Christian, Reiner Thomas, Kang Dongkyun
College of Optical Sciences, University of Arizona, Tucson, AZ 85721, USA.
These authors contributed equally to this work.
Biomed Opt Express. 2019 Dec 6;11(1):89-98. doi: 10.1364/BOE.11.000089. eCollection 2020 Jan 1.
Disease diagnosis in low-resource settings can be challenging due to the lack of equipment and trained personnel required for histologic analysis. In this paper, we have developed a smartphone-based epifluorescence microscope (SeFM) for imaging fresh tissues at sub-cellular resolution. SeFM provides similar resolution and field of view (FOV) as those used during histologic analysis. The SeFM device achieved the lateral resolution of 0.57 µm and provided microscopy images over a sample area larger than 500 µm. The material cost was low, approximately $3,000. Preliminary images of human pancreatic tumor specimens clearly visualized cellular details. Quantitative analysis showed that using an excess dose of a chemotherapy drug significantly reduced the tumor-specific fluorescence signal, confirming the specificity of the drug and the detection potential of SeFM.
由于缺乏组织学分析所需的设备和专业人员，在资源匮乏地区进行疾病诊断具有挑战性。在本文中，我们开发了一种基于智能手机的落射荧光显微镜（SeFM），用于在亚细胞分辨率下对新鲜组织进行成像。SeFM提供了与组织学分析中使用的分辨率和视野（FOV）相似的效果。SeFM设备实现了0.57 µm的横向分辨率，并在大于500 µm的样本区域上提供显微镜图像。材料成本很低，约为3000美元。人类胰腺肿瘤标本的初步图像清晰地显示了细胞细节。定量分析表明，使用过量剂量的化疗药物可显著降低肿瘤特异性荧光信号，证实了该药物的特异性以及SeFM的检测潜力。
