Wang Yi, Hu Yingxin, Peng Binyang, Zhou Hongxian, Zhao Yuqian, Ma Zhenhe
School of Control Engineering, Northeastern University at Qinhuangdao, Qinhuangdao 066004, China.
Experiment Education Center, Northeastern University at Qinhuangdao, Qinhuangdao 066004, China.
Biomed Opt Express. 2019 Dec 24;11(1):505-516. doi: 10.1364/BOE.381129. eCollection 2020 Jan 1.
We demonstrate a 3×3 coupler-based fiber-optic interferometric system to detect the local initial photoacoustic pressure. In contrast with the existing interferometric photoacoustic microscopy (PAM) relying on the measurement of the phase change of the probe light caused by the sample surface vibration, the present method measures the intensity change of the probe light caused by the initial photoacoustic pressure. Compared with the conventional interferometric PAMs, this method has the advantages: (1) it is free from the influence of the rough tissue surface, achieving complete noncontact in vivo imaging; (2) the probe light and the excitation light are focused at a same point below the sample surface, and the confocal configuration makes it more convenient for in vivo imaging; and (3) there is no need for phase stabilization, allowing a high imaging speed. These advantages show that the method will be a promising technique for in vivo imaging. This method is verified by imaging of a resolution test target and in vivo imaging of the blood vessels in a mouse ear.
我们展示了一种基于3×3耦合器的光纤干涉测量系统，用于检测局部初始光声压力。与现有的干涉光声显微镜（PAM）不同，现有方法依赖于测量由样品表面振动引起的探测光的相位变化，而本方法测量的是由初始光声压力引起的探测光的强度变化。与传统的干涉PAM相比，该方法具有以下优点：（1）不受粗糙组织表面的影响，实现了完全非接触式体内成像；（2）探测光和激发光聚焦在样品表面下方的同一点，共焦配置使其更便于进行体内成像；（3）无需相位稳定，成像速度高。这些优点表明该方法将是一种有前途的体内成像技术。通过对分辨率测试目标的成像和小鼠耳部血管的体内成像验证了该方法。
