Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, China.
Aging (Albany NY). 2020 Feb 6;12(3):2610-2625. doi: 10.18632/aging.102765.
Circular RNAs (circRNAs) have been reported to be involved in many diseases. But there is no report on circRNAs in non-obstructive azoospermia (NOA). The purpose of this paper is to explore the circular RNA expression profile and potential functions of circRNAs in NOA patients. We first preformed circRNA expression profiling analysis using a circRNA microarray in testicular samples from NOA and obstructive azoospermia (OA) patients. CircRNAs were validated by qRT-PCR. Bioinformatics analysis were used to construct the ceRNA network. GO and KEGG enrichment analysis were performed by using DAVID. Microarray analysis identified 82 differentially expressed circRNAs in NOA specimens. The differential expression of hsa_circRNA_402130, hsa_circRNA_072697, hsa_circRNA_030050, hsa_circRNA_100812 and hsa_circRNA_406168 was confirmed by qRT-PCR. Enrichment analysis revealed the association of hsa_circRNA_402130 and hsa_circRNA_072697 with multiple signaling pathways. The data indicated that circRNAs were significantly dysregulated in NOA specimens and might involve in the pathogenesis of NOA.
环状 RNA（circRNAs）已被报道参与多种疾病。但在非阻塞性无精子症（NOA）中尚无 circRNAs 的报道。本文旨在探讨环状 RNA 在 NOA 患者中的表达谱及潜在功能。我们首先使用环状 RNA 微阵列对来自 NOA 和阻塞性无精子症（OA）患者的睾丸组织样本进行环状 RNA 表达谱分析。通过 qRT-PCR 验证 circRNAs。通过 DAVID 进行 GO 和 KEGG 富集分析。微阵列分析鉴定出 82 个在 NOA 标本中差异表达的 circRNAs。hsa_circRNA_402130、hsa_circRNA_072697、hsa_circRNA_030050、hsa_circRNA_100812 和 hsa_circRNA_406168 的差异表达通过 qRT-PCR 得到了验证。富集分析表明 hsa_circRNA_402130 和 hsa_circRNA_072697 与多种信号通路有关。这些数据表明，circRNAs 在 NOA 标本中显著失调，可能参与了 NOA 的发病机制。
