School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.
Int J Mol Sci. 2020 Feb 4;21(3):1025. doi: 10.3390/ijms21031025.
() is a common foodborne pathogen that not only causes diseases and contaminates food, but also causes considerable economic losses. Therefore, it is necessary to find effective and feasible methods to control . In this study, changes in after treatment with benzyl isothiocyanate (BITC) were detected by transcriptomics to explore the antibacterial effect of BITC at subinhibitory concentration. The results showed that, in contrast to the control group (SC), the BITC-treated group (SQ_BITC) had 197 differentially expressed genes (DEGs), of which 115 were downregulated and 82 were upregulated. We screened out eight significantly downregulated virulence-related genes and verified gene expression by quantitative Real-time Polymerase Chain Reaction (qRT-PCR). We also selected motility and biofilm formation to observe the effects of BITC on the other virulence related factors of . The results showed that both swimming and swarming were significantly inhibited. BITC also had a significant inhibitory effect on biofilm formation, and showed an effect on bacterial morphology. These results will be helpful for understanding the mechanism of the antibacterial action of BITC against and other foodborne pathogens.
()是一种常见的食源性致病菌，不仅会引起疾病和污染食物，还会造成相当大的经济损失。因此，有必要寻找有效可行的方法来控制。在这项研究中，通过转录组学检测苄基异硫氰酸酯（BITC）处理后 ()的变化，探索亚抑菌浓度下 BITC 的抗菌作用。结果表明，与对照组（SC）相比，BITC 处理组（SQ_BITC）有 197 个差异表达基因（DEGs），其中 115 个下调，82 个上调。我们筛选出 8 个显著下调的毒力相关基因，并通过实时荧光定量聚合酶链反应（qRT-PCR）验证基因表达。我们还选择了运动性和生物膜形成来观察 BITC 对其他与毒力相关的因素的影响。结果表明，泳动和群集运动均受到显著抑制。BITC 对生物膜形成也有显著的抑制作用，并显示出对细菌形态的影响。这些结果将有助于理解 BITC 对和其他食源性致病菌的抗菌作用机制。
