Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Crop Foundation Division, National Institute of Crop Science, Rural Development Administration, Wanju, 55365, Republic of Korea.
Sci Rep. 2020 Feb 13;10(1):2588. doi: 10.1038/s41598-020-59518-x.
The sweet potato in the family Convolvulaceae is a dicotyledonous perennial plant. Here, we conducted a comprehensive sweet potato virome study using 10 different libraries from eight regions in Korea and two different sweet potato cultivars by RNA-Sequencing. Comprehensive bioinformatics analyses revealed 10 different virus species infecting sweet potato. Moreover, we identified two novel viruses infecting sweet potato referred to as Sweet potato virus E (SPVE) in the genus Potyvirus and Sweet potato virus F (SPVF) in the genus Carlavirus. Of the identified viruses, Sweet potato feathery mottle virus (SPFMV) was the dominant virus followed by Sweet potato virus C (SPVC) and SPVE in Korea. We obtained a total of 30 viral genomes for eight viruses. Our phylogenetic analyses showed many potyvirus isolates are highly correlated with geographical regions. However, two isolates of SPFMV and a single isolate of Sweet potato virus G (SPVG) were genetically distant from other known isolates. The mutation rate was the highest in SPFMV followed by SPVC and SPVG. Two different sweet potato cultivars, Beni Haruka and Hogammi, were infected by seven and five viruses, respectively. Taken together, we provide a complete list of viruses infecting sweet potato in Korea and diagnostic methods.
旋花科番薯属的番薯是一种双子叶多年生植物。在这里，我们通过 RNA 测序，对来自韩国八个地区和两个不同番薯品种的 10 个文库进行了全面的番薯病毒组研究。综合生物信息学分析揭示了 10 种不同的病毒感染番薯。此外，我们鉴定出两种新的感染番薯的病毒，分别命名为 Potyvirus 属的番薯病毒 E（SPVE）和 Carlavirus 属的番薯病毒 F（SPVF）。在所鉴定的病毒中，番薯羽状斑驳病毒（SPFMV）是韩国的优势病毒，其次是番薯病毒 C（SPVC）和 SPVE。我们共获得了 8 种病毒的 30 个病毒基因组。我们的系统发育分析表明，许多马铃薯 Y 病毒属的分离物与地理区域高度相关。然而，两个 SPFMV 分离物和一个单独的 SPVG 分离物在遗传上与其他已知分离物相距甚远。SPFMV 的突变率最高，其次是 SPVC 和 SPVG。两个不同的番薯品种 Beni Haruka 和 Hogammi 分别感染了 7 种和 5 种病毒。总之，我们提供了韩国感染番薯的完整病毒清单和诊断方法。
