组织微阵列在食管鳞状细胞癌中的应用。

Use of Tissue Microarray in Esophageal Squamous Cell Carcinoma.

机构信息

Cancer Molecular Pathology, School of Medicine, Griffith University, Gold Coast, Queensland, Australia.

Department of Biomedical Engineering, National Taiwan University, Taipei City, Taiwan.

出版信息

Methods Mol Biol. 2020;2129:119-135. doi: 10.1007/978-1-0716-0377-2_10.

DOI:10.1007/978-1-0716-0377-2_10

PMID:32056174

Abstract

Tissue microarray (TMA) is widely used for identifying the expression of markers in many tissues from patients with esophageal squamous cell carcinoma. The technology is mostly used in immunohistochemical studies to test the expression of markers and oncoproteins in signalling pathway as well as targeting proteins involved in therapies for esophageal squamous cell carcinoma. Appropriate use of TMA sections needs consideration of labor, planning, and expertise involved. For the best performance, it is important to design the layout of the TMA as well as use whole-slide scanning for interpretation of the TMA sections.

摘要

组织微阵列（TMA）广泛用于鉴定食管鳞癌患者多种组织中标志物的表达。该技术主要用于免疫组织化学研究，以检测信号通路中标志物和癌蛋白的表达以及参与食管鳞癌治疗的靶向蛋白。适当使用 TMA 切片需要考虑劳动力、规划和专业知识。为了获得最佳性能，设计 TMA 布局以及使用全切片扫描来解释 TMA 切片非常重要。