Department of Public Health, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8412, Japan.
Department of Biochemistry & Molecular Biology, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8412, Japan.
Fitoterapia. 2020 Apr;142:104524. doi: 10.1016/j.fitote.2020.104524. Epub 2020 Feb 21.
Five new cadinene-type sesquiterpenes, hibiscusterpenes I-V (1-5), and six known compounds (6-11) have been isolated from the methanol extract of the stems and the twigs of Hibiscus tiliaceus. The structures of the new compounds were elucidated based on chemical and spectroscopic evidence. The absolute stereochemistry of hibiscusterpene I (1) was determined using X-ray crystallography. For hibiscusterpene III (3), the absolute configuration was established upon comparison of the experimental and predicted electronic circular dichroism (ECD) data. Among the isolated compounds, hibiscone C (7) and syriacusin A (11) showed cytotoxic activity in HeLa cells. In addition, their cell death-inducing activity was observed using time-lapse cell imaging.
从黄槿的茎和小枝甲醇提取物中分离得到了五个新的卡丹烯型倍半萜类化合物，即 hibiscusterpenes I-V（1-5），以及六个已知化合物（6-11）。根据化学和光谱证据阐明了新化合物的结构。通过 X 射线晶体学确定了 hibiscusterpene I（1）的绝对立体化学。对于 hibiscusterpene III（3），通过比较实验和预测的电子圆二色性（ECD）数据确定了其绝对构型。在所分离的化合物中，hibiscone C（7）和 syriacusin A（11）在 HeLa 细胞中表现出细胞毒性。此外，还通过延时细胞成像观察到它们诱导细胞死亡的活性。
