Moghadam Mohsen Rezaei, Jafarinasab Mohammad-Reza, Yousefi Zahra, Moghaddam Ali Sanjari, Memarzadeh Hajar, Kanavi Mozhgan Rezaei
Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Torfeh Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Ophthalmic Vis Res. 2020 Feb 2;15(1):7-15. doi: 10.18502/jovr.v15i1.5932. eCollection 2020 Jan-Mar.
To investigate the efficacy of topical (AV) gel-derived eye drops on the healing of alkali-burned corneas in rabbits.
Thirty alkali-burned corneas of 30 New Zealand albino rabbits were categorized into three groups: AV treatment group that received AV gel-derived eye drops four times a day; medical therapy (MT) group that received conventional treatment; and the control group. Clinical examinations together with digital imaging of the corneas were performed on days 0, 1, 2, 4, and 7. The area of the corneal epithelial defect (CED) was measured using ImageJ software. After euthanizing the rabbits, the affected corneas were evaluated by histopathological examination. Finally, the clinical and histopathological results were compared among the groups.
The CED area on days 2 and 7 was significantly less in the AV group than that in the MT group ( = 0.007 and = 0.024, respectively) and the control group ( = 0.003 and = 0.037, respectively). None of the cases developed hypersensitivity reactions, limbal ischemia, descemetocele, or corneal perforation during the study period. Based on histopathology, the AV group had notably less keratocyte loss than the MT group ( = 0.001) and the control group ( = 0.022). The inflammatory response after the alkali burn was higher in the AV group than that in the controls ( = 0.028).
Short-term topical AV treatment was effective in healing alkali-burned corneas and hastened corneal re-epithelialization as compared to MT; however, AV gel-derived eye drops did not reduce the inflammatory response.
研究局部应用（AV）凝胶衍生眼药水对兔碱烧伤角膜愈合的疗效。
将30只新西兰白化兔的30只碱烧伤角膜分为三组：AV治疗组，每天接受4次AV凝胶衍生眼药水；药物治疗（MT）组，接受常规治疗；以及对照组。在第0、1、2、4和7天进行临床检查并对角膜进行数字成像。使用ImageJ软件测量角膜上皮缺损（CED）的面积。对兔子实施安乐死后，通过组织病理学检查评估受影响的角膜。最后，比较各组的临床和组织病理学结果。
AV组在第2天和第7天的CED面积明显小于MT组（分别为P = 0.007和P = 0.024）和对照组（分别为P = 0.003和P = 0.037）。在研究期间，无一例出现过敏反应、角膜缘缺血、后弹力层膨出或角膜穿孔。基于组织病理学，AV组的角膜细胞损失明显少于MT组（P = 0.001）和对照组（P = 0.022）。碱烧伤后的炎症反应在AV组高于对照组（P = 0.028）。
与MT相比，短期局部应用AV治疗对碱烧伤角膜的愈合有效，并加速了角膜再上皮化；然而，AV凝胶衍生眼药水并未减轻炎症反应。
