Thenmozhi T
Department of Biotechnology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh 517 502 India.
3 Biotech. 2020 Feb;10(2):82. doi: 10.1007/s13205-020-2088-7. Epub 2020 Feb 1.
It has been reported that iron oxide nanoparticles have various biomedical applications, including cancer diagnosis and treatment. Iron oxide nanoparticles were known to exert cytotoxicity against MCF-7 breast cancer cell lines and in this present study, we have investigated for their apoptosis-inducing potential in the same cell line. The flow cytometry analysis of the MCF-7 breast cancer cell lines treated with functionalized iron oxide nanoparticles showed an increased percentage of cells in terms of viable, early, and late apoptosis. The cell cycle analysis of the MCF-7 cell lines treated with extract coated with polyvinylpyrrolidone (PVP) + iron oxide nanoparticles and PVP + iron oxide nanoparticles showed substantial accumulation of nanoparticles in the sub-G1 phase, confirming induction of apoptosis. The activities of caspase-3, caspase-8, and caspase-9 increased with increasing concentration of the nanoparticles indicating that activities of caspase can be activated by iron nanoparticles. Further, functionalized nanoparticles induced oxidative stress through reactive oxygen species (ROS) formation. Therefore, it is concluded that the functionalized iron nanoparticles induce apoptosis in MCF-7 breast cancer cell lines and further provides an opportunity to explore the iron nanoparticles for apoptosis in cancer treatment.
据报道，氧化铁纳米颗粒具有多种生物医学应用，包括癌症诊断和治疗。已知氧化铁纳米颗粒对MCF-7乳腺癌细胞系具有细胞毒性，在本研究中，我们研究了它们在同一细胞系中的凋亡诱导潜力。用功能化氧化铁纳米颗粒处理的MCF-7乳腺癌细胞系的流式细胞术分析显示，在存活、早期和晚期凋亡方面，细胞百分比增加。用涂有聚乙烯吡咯烷酮（PVP）+氧化铁纳米颗粒和PVP+氧化铁纳米颗粒的提取物处理的MCF-7细胞系的细胞周期分析显示，纳米颗粒在亚G1期大量积累，证实了凋亡的诱导。随着纳米颗粒浓度的增加，半胱天冬酶-3、半胱天冬酶-8和半胱天冬酶-9的活性增加，表明铁纳米颗粒可以激活半胱天冬酶的活性。此外，功能化纳米颗粒通过活性氧（ROS）的形成诱导氧化应激。因此，得出结论，功能化铁纳米颗粒在MCF-7乳腺癌细胞系中诱导凋亡，并进一步为探索铁纳米颗粒在癌症治疗中的凋亡作用提供了机会。
