College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, Shandong, People's Republic of China.
Arch Microbiol. 2023 Feb 2;205(2):79. doi: 10.1007/s00203-023-03408-z.
A Gram-staining-positive, heterotrophic, non-spore-forming, non-motile, rod-shaped, strain ZS14-85 belonging to the genus Cryobacterium was isolated from soil in Antarctica. Growth was observed in the presence of 0-2% (w/v) NaCl, at pH 7.0-9.0 (optimum, pH 7.0) and 4-30 ℃ (optimum, 20 ℃). Phylogenetic analysis showed that strain ZS14-85 formed a lineage in the genus Cryobacterium. The digital DNA-DNA hybridization (dDDH) values between strain ZS14-85 and its close relatives Cryobacterium psychrotolerans CGMCC 1.5382, Cryobacterium soli MCCC 1K03549 and Cryobacterium breve NBRC 113800 were 22.5, 22.3 and 22.2%, respectively. Orthologous Average Nucleotide Identity (OrthoANI) scores between strain ZS14-85 and C. psychrotolerans CGMCC 1.5382, C. breve NBRC 113800 and C. soli MCCC 1K03549 were 78.7, 78.1 and 77.7%, respectively. The polar lipids of strain ZS14-85 were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), one unidentified glycolipid (GL) and two unidentified lipids (L). The major fatty acids were anteiso-C (60.7%), iso-C (17.0%) and anteiso-C (15.2%). MK-10, MK-11 and MK-9 were the predominant respiratory menaquinones. Based on phenotypic, phylogenetic and genotypic data, a novel species, Cryobacterium zhongshanensis sp. nov. is proposed. The type strain is ZS14-85 (= CCTCC AB 2019396 = KCTC 49384).
一株革兰氏阳性、异养、非孢子形成、非运动、杆状的菌株 ZS14-85 从南极洲土壤中分离得到，属于 Cryobacterium 属。该菌在 0-2%（w/v）NaCl、pH7.0-9.0（最佳 pH7.0）和 4-30℃（最佳 20℃）条件下生长。系统发育分析表明，菌株 ZS14-85 在 Cryobacterium 属中形成一个谱系。菌株 ZS14-85 与其近缘种 Cryobacterium psychrotolerans CGMCC 1.5382、Cryobacterium soli MCCC 1K03549 和 Cryobacterium breve NBRC 113800 的数字 DNA-DNA 杂交（dDDH）值分别为 22.5%、22.3%和 22.2%。菌株 ZS14-85 与 C. psychrotolerans CGMCC 1.5382、C. breve NBRC 113800 和 C. soli MCCC 1K03549 的 OrthoANI 得分分别为 78.7%、78.1%和 77.7%。菌株 ZS14-85 的极性脂为双磷脂酰甘油（DPG）、磷脂酰甘油（PG）、一种未鉴定的糖脂（GL）和两种未鉴定的脂（L）。主要脂肪酸为 anteiso-C（60.7%）、iso-C（17.0%）和 anteiso-C（15.2%）。MK-10、MK-11 和 MK-9 是主要的呼吸menaquinones。基于表型、系统发育和基因型数据，提出了一个新种，即Cryobacterium zhongshanensis sp. nov.。模式菌株为 ZS14-85（= CCTCC AB 2019396 = KCTC 49384）。
