Cryobacterium zhongshanensis sp. nov., an actinobacterium isolated from Antarctic soil.

A Gram-staining-positive, heterotrophic, non-spore-forming, non-motile, rod-shaped, strain ZS14-85 belonging to the genus Cryobacterium was isolated from soil in Antarctica. Growth was observed in the presence of 0-2% (w/v) NaCl, at pH 7.0-9.0 (optimum, pH 7.0) and 4-30 ℃ (optimum, 20 ℃). Phylogenetic analysis showed that strain ZS14-85 formed a lineage in the genus Cryobacterium. The digital DNA-DNA hybridization (dDDH) values between strain ZS14-85 and its close relatives Cryobacterium psychrotolerans CGMCC 1.5382, Cryobacterium soli MCCC 1K03549 and Cryobacterium breve NBRC 113800 were 22.5, 22.3 and 22.2%, respectively. Orthologous Average Nucleotide Identity (OrthoANI) scores between strain ZS14-85 and C. psychrotolerans CGMCC 1.5382, C. breve NBRC 113800 and C. soli MCCC 1K03549 were 78.7, 78.1 and 77.7%, respectively. The polar lipids of strain ZS14-85 were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), one unidentified glycolipid (GL) and two unidentified lipids (L). The major fatty acids were anteiso-C (60.7%), iso-C (17.0%) and anteiso-C (15.2%). MK-10, MK-11 and MK-9 were the predominant respiratory menaquinones. Based on phenotypic, phylogenetic and genotypic data, a novel species, Cryobacterium zhongshanensis sp. nov. is proposed. The type strain is ZS14-85 (= CCTCC AB 2019396 = KCTC 49384).