LINC01410基因敲低通过靶向miR-2467-3p/VOPP1轴抑制宫颈癌的生长和侵袭。
LINC01410 Knockdown Suppresses Cervical Cancer Growth and Invasion via Targeting miR-2467-3p/VOPP1 Axis.
作者信息
Liu Fengjuan, Wen Chuansong
机构信息
Department of Gynecology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, People's Republic of China.
出版信息
Cancer Manag Res. 2020 Feb 5;12:855-861. doi: 10.2147/CMAR.S236832. eCollection 2020.
BACKGROUND
Long noncoding RNAs have essential roles in human diseases, including cancer. Our work aims to assess the function and mechanisms of LINC01410 in cervical cancer (CC) development.
METHODS
Expression analyses were performed using qRT-PCR. Proliferation was determined through CCK8 and colony formation assays. Cell migration and invasion were determined by Transwell assay. The interactions among LINC01410, miR-2467-3p and VOPP1 were analyzed via luciferase reporter assay.
RESULTS
LINC01410 was upregulated in CC tissues and cell lines. LINC01410 upregulation correlated with poor prognosis. LINC01410 silencing suppressed proliferation, migration and invasion of CC cells. LINC01410 was the sponge for miR-2467. And LINC01410 promoted VOPP1 expression through inhibiting miR-2467.
CONCLUSION
Our findings demonstrated that LINC01410 contributed to CC progression through regulating miR-2467/VOPP1 axis and suggested that LINC01410/miR-2467/VOPP1 cascade may be a potential therapeutic target.
背景
长链非编码RNA在包括癌症在内的人类疾病中发挥着重要作用。我们的工作旨在评估LINC01410在宫颈癌（CC）发展中的功能和机制。
方法
使用qRT-PCR进行表达分析。通过CCK8和集落形成试验确定增殖情况。通过Transwell试验确定细胞迁移和侵袭情况。通过荧光素酶报告基因试验分析LINC01410、miR-2467-3p和VOPP1之间的相互作用。
结果
LINC01410在CC组织和细胞系中上调。LINC01410上调与预后不良相关。LINC01410沉默抑制了CC细胞的增殖、迁移和侵袭。LINC01410是miR-2467的海绵。并且LINC01410通过抑制miR-2467促进VOPP1表达。
结论
我们的研究结果表明，LINC01410通过调节miR-2467/VOPP1轴促进CC进展，并表明LINC01410/miR-2467/VOPP1级联可能是一个潜在的治疗靶点。
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