School of Food and Bioengineering, Hezhou University, Hezhou 542899, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin 541004, China.
School of Food and Bioengineering, Hezhou University, Hezhou 542899, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin 541004, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 May 5;232:118174. doi: 10.1016/j.saa.2020.118174. Epub 2020 Feb 19.
Here, Au-doped carbon dots (CD) nanosols with good stability were prepared by hydrothermal reaction method. We found that CD can efficiently catalyze the nanoreaction of reducing AgNO by glucose, and at 420 nm，the reaction products of yellow spherical silver nanosol exhibit an intense surface plasmon resonance (SPR) absorption peak. The nucleic acid aptamers (Apt) can be adsorbed on the surface of carbon dots, so that their catalytic activity was suppressed, the nanosilvers were reduced, the solution color becomes lighter, and the Abs value was weakened. When As was added, it forms a stable conjugate with the Apt, releases free carbon dots, restored its catalytic activity, and the color and Abs signals enhanced linearly. Based on the Apt regulation and the catalytic amplification effect of CD on AgNO-glucose, a new extremely sensitive SPR spectrophotometric method for the determination of arsenic ion content of 0.025-0.75 μg/L was established, and the detection limit of As is 0.01 μg/L.
在这里，通过水热反应法制备了具有良好稳定性的 Au 掺杂碳点（CD）纳米溶胶。我们发现 CD 可以有效地催化葡萄糖还原 AgNO 的纳米反应，在 420nm 处，黄色球形银纳米溶胶的反应产物表现出强烈的表面等离子体共振（SPR）吸收峰。核酸适体（Apt）可以吸附在碳点表面，从而抑制其催化活性，还原纳米银，溶液颜色变浅，Abs 值减弱。当加入 As 时，它与 Apt 形成稳定的配合物，释放出游离的碳点，恢复其催化活性，颜色和 Abs 信号线性增强。基于 Apt 的调节和 CD 对 AgNO-葡萄糖的催化放大效应，建立了一种新的、极其灵敏的 SPR 分光光度法，用于测定 0.025-0.75μg/L 范围内的砷离子含量，检测限为 0.01μg/L。
