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富含血小板血浆组织愈合相关蛋白的蛋白质组学分类和鉴定。

Proteomic Classification and Identification of Proteins Related to Tissue Healing of Platelet-Rich Plasma.

机构信息

Department of Orthopaedic Surgery, Hallym University Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea.

Gachon Medical Research Institute, Gil Medical Center, Gachon University, Incheon, Korea.

出版信息

Clin Orthop Surg. 2020 Mar;12(1):120-129. doi: 10.4055/cios.2020.12.1.120. Epub 2020 Feb 13.

DOI:10.4055/cios.2020.12.1.120
PMID:32117548
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7031431/
Abstract

BACKGROUND

Platelet-rich plasma (PRP) is a plasma component of autologous blood containing a high concentration of platelets. PRP is used to promote healing of damaged tissues. However, there are not many studies on the composition and expression patterns of active proteins in PRP. The purpose of this study was to identify unknown factors that contribute to tissue healing by proteomic analysis of proteins in PRP.

METHODS

Three men in their 30s with no basal disease participated in this study. All identified proteins were classified for tissue healing-related functions on the basis of the gene ontology analysis of adhesion molecule with Ig-like domain 2 (AmiGO2). PRP was prepared by using the ACP kit and GPS III kit.

RESULTS

We identified a total of 125 proteins related to wound healing, along with three proteins for angiogenesis involved in wound healing, two proteins for fibroblast migration, four proteins for collagen biosynthesis process, two proteins for glycosaminoglycan biosynthesis process, and 13 proteins for glycosaminoglycan binding. So, in addition to the growth factors that have been already known to be involved in tissue healing, 25 new proteins were identified.

CONCLUSIONS

We identified the unknown proteins associated with tissue healing in PRP. Our findings may serve as a foundation for the establishment of basic medical evidence for PRP applications.

摘要

背景

富含血小板的血浆 (PRP) 是自体血液的血浆成分，含有高浓度的血小板。PRP 用于促进受损组织的愈合。然而，关于 PRP 中活性蛋白的组成和表达模式的研究并不多。本研究旨在通过对 PRP 中的蛋白质进行蛋白质组学分析，鉴定出有助于组织愈合的未知因素。

方法

本研究纳入了 3 名年龄在 30 多岁、无基础疾病的男性。所有鉴定出的蛋白质均根据粘附分子 Ig 样结构域 2（AmiGO2）的基因本体分析进行了与组织愈合相关功能的分类。使用 ACP 试剂盒和 GPS III 试剂盒制备 PRP。

结果

我们共鉴定出 125 种与伤口愈合相关的蛋白质，其中涉及伤口愈合的血管生成有 3 种蛋白、2 种成纤维细胞迁移蛋白、4 种胶原蛋白生物合成过程蛋白、2 种糖胺聚糖生物合成过程蛋白和 13 种糖胺聚糖结合蛋白。因此，除了已知与组织愈合有关的生长因子外，还鉴定出了 25 种新的蛋白质。

结论

我们鉴定出了 PRP 中与组织愈合相关的未知蛋白。我们的研究结果可能为 PRP 应用的基础医学证据的建立提供依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fef/7031431/a8898c13ed57/cios-12-120-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fef/7031431/04c1ee5086e3/cios-12-120-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fef/7031431/1ae0ac72f455/cios-12-120-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fef/7031431/a8898c13ed57/cios-12-120-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fef/7031431/04c1ee5086e3/cios-12-120-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fef/7031431/1ae0ac72f455/cios-12-120-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fef/7031431/a8898c13ed57/cios-12-120-g003.jpg

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