Al-Eitan Laith N, Tarkhan Amneh H, Alghamdi Mansour A, Al-Qarqaz Firas A, Al-Kofahi Hadeel S
Department of Applied Biological Sciences, Jordan University of Science and Technology, Irbid, 22110, Jordan.
Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid, 22110, Jordan.
BMC Med Genomics. 2020 Mar 9;13(1):35. doi: 10.1186/s12920-020-0700-7.
The human papillomaviruses (HPV) are a group of viruses that, depending on the strain, can cause cancer or the formation of benign growths known as warts. Scarce information exists with regard to the genetic nature of non-genital cutaneous warts induced by the human papillomavirus (HPV).
The main purpose of this study is to investigate the differences between the gene expression profiles of common warts and healthy skin in HPV-positive individuals by RNA sequencing on the Illumina HiSeq 2500. After obtaining shave biopsies of common warts and healthy skin from twelve Arab males, we were able to analyze the transcriptomes of 24 paired cases and controls.
Common warts were found to possess a highly significant and unique molecular signature. Many of the most up-regulated (KRT16, EPGN, and ABCG4) and down-regulated genes (C15orf59, CYB561A3, and FCGRT) in warts were the subject of little investigation in the published literature. Moreover, the top 500 differentially expressed genes were found to be associated with immune and autoimmune pathways, such as the neutrophil degranulation, toll-like receptor 7/8 (TLR 7/8) cascade, toll-like receptor 9 (TLR9) cascade, and toll-like receptor 10 (TLR10) pathways, among others.
Our findings are particularly important because they serve as the most comprehensive to date with regard to the modulation of human skin gene expression by HPV infection.
人乳头瘤病毒（HPV）是一组病毒，根据毒株不同，可导致癌症或形成称为疣的良性生长物。关于人乳头瘤病毒（HPV）引起的非生殖器皮肤疣的遗传性质，现有信息稀少。
本研究的主要目的是通过在Illumina HiSeq 2500上进行RNA测序，调查HPV阳性个体中寻常疣和健康皮肤的基因表达谱差异。从12名阿拉伯男性身上获取寻常疣和健康皮肤的剃刀活检样本后，我们能够分析24对病例和对照的转录组。
发现寻常疣具有高度显著且独特的分子特征。疣中许多上调幅度最大的基因（角蛋白16、表皮生长因子前体、ATP结合盒转运体G4）和下调基因（15号染色体开放阅读框59、细胞色素b561A3、Fc片段受体重链）在已发表的文献中很少被研究。此外，发现前500个差异表达基因与免疫和自身免疫途径相关，如中性粒细胞脱颗粒、Toll样受体7/8（TLR 7/8）级联、Toll样受体9（TLR9）级联和Toll样受体10（TLR10）途径等。
我们的发现尤为重要，因为就HPV感染对人类皮肤基因表达的调节而言，它们是迄今为止最全面的。
