Research and Development, Corteva Agriscience, Johnston, IA, USA.
Bill & Melinda Gates foundation, Seattle, WA, USA.
Nat Biotechnol. 2020 May;38(5):579-581. doi: 10.1038/s41587-020-0444-0. Epub 2020 Mar 9.
We created waxy corn hybrids by CRISPR-Cas9 editing of a waxy allele in 12 elite inbred maize lines, a process that was more than a year faster than conventional trait introgression using backcrossing and marker-assisted selection. Field trials at 25 locations showed that CRISPR-waxy hybrids were agronomically superior to introgressed hybrids, producing on average 5.5 bushels per acre higher yield.
我们通过 CRISPR-Cas9 编辑技术对 12 个优秀自交系玉米品系中的一个蜡质等位基因进行了修饰,从而创造出了蜡质玉米杂交种。与传统的回交和标记辅助选择方法相比,这一过程的速度要快一年多。在 25 个地点进行的田间试验表明,CRISPR 蜡质杂交种在农艺性状上优于导入杂交种,平均每亩产量高出 5.5 蒲式耳。