Wang Maoqing, Chen Hongyu, Du Shanshan, Guo Xinxin, Zhao Jiali, Sun Changhao, Li Ying
National Key Disciplines of Nutrition and Food Hygiene, Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, 157 Baojian Road, Nangang District, Harbin 150081 People's Republic of China.
BMC Nutr. 2018 Mar 27;4:12. doi: 10.1186/s40795-018-0219-x. eCollection 2018.
The aims of the current study were to assess the nutritional status of 25OHD3 and retinol in a northern Chinese population using our established reliable method for the simultaneous determination of serum 25OHD3 and retinol.
We established a reliable method for the simultaneous determination of 25OHD3 and retinol using SPE and UPLC/PDA; measured the serum levels of 25OHD3 and retinol in elementary school students, middle school students, and adults ( = 1181) in northern China; and assessed their nutritional status.
Our method had good precision, detection limit, and linear quantitative range and could process 100 samples within 12 h. The average levels of 25OHD3 and retinol were 16.1 ± 6.7 ng/ml and 328.1 ± 117.1 ng/ml, respectively, in all samples. VD deficiency was common, with a prevalence > 60% in all three age groups, and the high prevalence of VA deficiency (26.1%) was observed only in the elementary school students.
Vitamin A supplementation should be considered for elementary school students, and vitamin D supplementation is highly recommended for all age groups in Harbin. Our method could be widely adopted in population-based studies and clinical practice.
本研究旨在运用我们建立的同时测定血清25羟维生素D3（25OHD3）和视黄醇的可靠方法，评估中国北方人群中25OHD3和视黄醇的营养状况。
我们建立了一种使用固相萃取（SPE）和超高效液相色谱/光电二极管阵列检测法（UPLC/PDA）同时测定25OHD3和视黄醇的可靠方法；测定了中国北方小学生、中学生和成年人（n = 1181）的血清25OHD3和视黄醇水平，并评估了他们的营养状况。
我们的方法具有良好的精密度、检测限和线性定量范围，并且能够在12小时内处理100个样本。所有样本中25OHD3和视黄醇的平均水平分别为16.1±6.7 ng/ml和328.1±117.1 ng/ml。维生素D缺乏情况普遍，在所有三个年龄组中患病率均>60%，而维生素A缺乏的高患病率（26.1%）仅在小学生中观察到。
应考虑对小学生补充维生素A，强烈建议哈尔滨市所有年龄组补充维生素D。我们的方法可广泛应用于基于人群的研究和临床实践。
