Ovsepian Saak V, Jiang Yuanyuan, Sardella Thomas C P, Malekzadeh-Najafabadi Jaber, Burton Neal C, Yu Xin, Ntziachristos Vasilis
Institute for Biological and Medical Imaging, Helmholtz Zentrum Munich, German Research Center for Environmental Health, Neuherberg, Germany.
Munich School of Bioengineering and Chair of Biological Imaging at Technical University Munich, Munich, Germany.
Photoacoustics. 2019 Dec 26;17:100153. doi: 10.1016/j.pacs.2019.100153. eCollection 2020 Mar.
To date, the vast majority of intra-vital neuroimaging systems applied in clinic and diagnostics is stationary with a rigid scanning element, requires specialized facilities and costly infrastructure. Here, we describe a simple yet radical approach for optoacoustic (photoacoustic) brain imaging using a light-weight handheld probe. It enables multispectral video-rate visualization of hemoglobin gradient changes in the cortex of adult rats induced by whisker and forelimb sensory inputs, as well as by optogenetic stimulation of intra-cortical connections. With superb penetration and molecular specificity, described here in method holds major promises for future applications in research, routine ambulatory neuroimaging, and diagnostics.
迄今为止，临床上应用于诊断的绝大多数活体神经成像系统都是固定的，带有刚性扫描元件，需要专门的设备和昂贵的基础设施。在此，我们描述了一种使用轻型手持探头进行光声脑成像的简单而全新的方法。它能够以多光谱视频速率可视化成年大鼠皮层中由触须和前肢感觉输入以及皮层内连接的光遗传学刺激引起的血红蛋白梯度变化。本文所述方法具有出色的穿透性和分子特异性，在未来的研究、常规动态神经成像和诊断应用中具有重大前景。
