Academy of Scientific and Innovative Research (AcSIR), CSIR-CFTRI Campus, Mysuru, Karnataka, India.
Department of Biochemistry, CSIR-CFTRI, Mysuru, Karnataka, India.
Sci Rep. 2020 Mar 10;10(1):4456. doi: 10.1038/s41598-020-61358-8.
Edible nanoparticles (ENPs) are nano-sized vesicles derived from edible plants. These ENPs are loaded with plant derived microRNAs, protein, lipids and phytochemicals. Recently, ginger derived ENPs was shown to prevent inflammatory bowel diseases and colon cancer, in vivo, highlighting their therapeutic potential. Conventionally, differential centrifugation with an ultra-centrifugation step is employed to purify these ENPs which imposes limitation on the cost-effectiveness of their purification. Herein, we developed polyethylene glycol-6000 (PEG6000) based ginger ENP purification (PEG-ENPs) method, which eliminates the need for expensive ultracentrifugation. Using different PEG6000 concentrations, we could recover between 60% to 90% of ENPs compared to ultracentrifugation method. PEG-ENPs exhibit near identical size and zeta potential similar to ultra-ENPs. The biochemical composition of PEG-ENPs, such as proteins, lipids, small RNAs and bioactive content is comparable to that of ultra-ENPs. In addition, similar to ultra-ENPs, PEG-ENPs are efficiently taken up by the murine macrophages and protects cells from hydrogen peroxide induced oxidative stress. Since PEG has been approved as food additive, the PEG method described here will provide a cost-effective alternative to purify ENPs, which can be directly used as a dietary supplement in therapeutic formulations.
可食用纳米颗粒（ENPs）是源自食用植物的纳米大小的囊泡。这些 ENPs 负载有植物衍生的 microRNAs、蛋白质、脂质和植物化学物质。最近，姜衍生的 ENPs 被证明可以预防炎症性肠病和结肠癌，在体内，突出了它们的治疗潜力。传统上，采用差速离心与超速离心步骤来纯化这些 ENPs，这对其纯化的成本效益施加了限制。在此，我们开发了基于聚乙二醇-6000（PEG6000）的姜 ENP 纯化（PEG-ENPs）方法，该方法消除了对昂贵的超速离心的需求。使用不同的 PEG6000 浓度，与超速离心法相比，我们可以回收 60%到 90%的 ENPs。PEG-ENPs 表现出与超 ENPs 相似的粒径和 ζ 电位。PEG-ENPs 的生化成分，如蛋白质、脂质、小 RNA 和生物活性成分，与超 ENPs 相当。此外，与超 ENPs 相似，PEG-ENPs 被小鼠巨噬细胞有效摄取，并保护细胞免受过氧化氢诱导的氧化应激。由于 PEG 已被批准为食品添加剂，因此此处描述的 PEG 方法将为纯化 ENPs 提供一种具有成本效益的替代方法，可直接用作治疗制剂中的膳食补充剂。
