College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China.
College of Engineering, Huazhong Agricultural University, Wuhan 430070, China.
Carbohydr Polym. 2020 May 15;236:116090. doi: 10.1016/j.carbpol.2020.116090. Epub 2020 Feb 28.
The present study aimed at the design and fabrication of a novel co-delivery system to encapsulate and protect curcumin and resveratrol. Curcumin and resveratrol had synergistic physiological activities when used together, but it was challenging to co-encapsulate them in traditional delivery vehicles due to their difference in molecular polarity. The curcumin-zein-resveratrol-chitosan nanocomplexes were developed and their formation mechanism and physicochemical properties were investigated. Hydrogen bonding and hydrophobic effects were dominant binding forces to stabilize the nanocomplexes. As the relative molecular weight of chitosan was increased, the mean size and surface positive charge of the nanocomplexes were elevated. The optimized nanocomplex based on zein and low molecular weight chitosan (50000-190000 Da) had a relatively high encapsulation efficiency of curcumin (91.3 %) and resveratrol (82.1 %), as well as exhibited the best physical stability. In addition, the half-life times of curcumin and resveratrol in the nanocomplex were extended by 4.5- and 1.9-fold during photodegradation, and the retention rates were increased by 3.7- and 1.1-fold than their free forms during thermal degradation. The nanocomplexes could also remain stable and delay the degradation of both bioactive compounds during long term storage. In summary, the nanocomplex showed to be an effective vehicle for the co-delivery of synergistic nutraceuticals.
本研究旨在设计和制备一种新型共递药系统，以封装和保护姜黄素和白藜芦醇。姜黄素和白藜芦醇一起使用时具有协同的生理活性，但由于它们的分子极性不同，将它们共同包封在传统的递药载体中具有挑战性。开发了姜黄素-玉米醇溶蛋白-白藜芦醇-壳聚糖纳米复合物，并研究了其形成机制和理化性质。氢键和疏水作用是稳定纳米复合物的主要结合力。随着壳聚糖相对分子量的增加，纳米复合物的平均粒径和表面正电荷增加。基于玉米醇溶蛋白和低分子量壳聚糖（50000-190000 Da）的优化纳米复合物对姜黄素（91.3%）和白藜芦醇（82.1%）的包封效率较高，具有较好的物理稳定性。此外，在光降解过程中，纳米复合物中姜黄素和白藜芦醇的半衰期分别延长了 4.5 倍和 1.9 倍，在热降解过程中，保留率分别比它们的游离形式增加了 3.7 倍和 1.1 倍。纳米复合物在长期储存过程中也能保持稳定，延缓两种生物活性化合物的降解。总之，该纳米复合物显示出作为协同营养药物共递药的有效载体。
