Setiyono Edi, Adhiwibawa Marcelinus Alfasisurya Setya, Indrawati Renny, Prihastyanti Monika Nur Utami, Shioi Yuzo, Brotosudarmo Tatas Hardo Panintingjati
Ma Chung Research Center for Photosynthetic Pigments (MRCPP) and Department of Chemistry, Universitas Ma Chung, Villa Puncak Tidar N01, Malang 65151, Jawa Timur, Indonesia.
ACS Omega. 2020 Feb 26;5(9):4626-4635. doi: 10.1021/acsomega.9b04322. eCollection 2020 Mar 10.
Red pigmented marine bacteria, strains PS1 and SB14, were isolated from two sampling locations in different ecosystems on Alor Island, Indonesia, and cultured in the laboratory. We analyzed the 16S rRNA gene sequences and examined the pigment composition and found that both strains produced cycloprodigiosin (), prodigiosin (), and 2-methyl-3-hexyl-prodiginine () as major compounds. In addition, we detected three minor compounds: prodigiosin derivatives 2-methyl-3-propyl prodiginine (), 2-methyl-3-butyl prodiginine (), and 2-methyl-3-heptyl-prodiginine (). To our knowledge, this is the first report that synthesizes not only prodigiosin and cycloprodigiosin but also four prodigiosin derivatives that differ in the length of the alkyl chain. The antimicrobial activity of cycloprodigiosin, prodigiosin, and 2-methyl-3-hexyl-prodiginine was examined by a disk-diffusion test against , , , and . We found that, at a concentration of 20 μg/mL, cycloprodigiosin showed the greatest inhibition (25.1 ± 0.55 mm) against .
红色海洋细菌PS1和SB14菌株，是从印度尼西亚阿洛岛不同生态系统的两个采样点分离得到，并在实验室中进行培养。我们分析了16S rRNA基因序列，检测了色素组成，发现这两种菌株均产生环丙基灵菌红素、灵菌红素和2-甲基-3-己基灵菌红素作为主要化合物。此外，我们还检测到三种次要化合物：灵菌红素衍生物2-甲基-3-丙基灵菌红素、2-甲基-3-丁基灵菌红素和2-甲基-3-庚基灵菌红素。据我们所知，这是首次报道不仅能合成灵菌红素和环丙基灵菌红素，还能合成四种烷基链长度不同的灵菌红素衍生物。通过纸片扩散法检测了环丙基灵菌红素、灵菌红素和2-甲基-3-己基灵菌红素对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和白色念珠菌的抗菌活性。我们发现，在浓度为20μg/mL时，环丙基灵菌红素对金黄色葡萄球菌的抑制作用最强（25.1±0.55mm）。
