Oh Won-Seok, Jung Jae-Chul, Choi Yong-Min, Mun Ju-Young, Ku Sae-Kwang, Song Chang-Hyun
Department of Veterinary Internal Medicine College of Veterinary Medicine Kyungpook National University Daegu Korea.
Life Science Research Institute NOVAREX CO., Ltd. Cheongju Korea.
Food Sci Nutr. 2020 Feb 14;8(3):1718-1728. doi: 10.1002/fsn3.1460. eCollection 2020 Mar.
Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD), characterized by the gut mucosal ulceration. Growing evidence indicates that dysregulation of immune response to the commensal microbiota involves the pathogenesis of IBD. Previous studies have demonstrated the favorable probiotic effects of fermented rice extracts through triple fermentation with and (FRe). Thus, the therapeutic potential of FRe for UC was examined. Dextran sodium sulfate UC mice model was orally administered distilled water as a control, sulfasalazine, or FRe at 300, 200, and 100 mg/kg, once a day for a week. The UC control exhibited body weight loss, bloody stools, and colonic shortening. However, the FRe, especially at 300 mg/kg, led to a reduction in weight loss, disease activity index scores, and colon weight, and an increase in colorectal length. The histopathological analyses revealed mild changes involved in the colonic crypt and mucosal damages in the FRe groups, along with inhibited inflammation. Indeed, the FRe reduced neutrophil infiltration and production of proinflammatory cytokines (i.e., tumor necrosis factor-α, interleukin-6/-8). This was accompanied by the down-regulation of nuclear factor-kappa B. The gene expression responsible for the intestinal barrier integrity (i.e., /, , , /) was up-regulated in the FRe groups. In addition, the FRe reduced lipid peroxidation and enhanced antioxidant activity. Interestingly, the microbiota dysbiosis was attenuated in the FRe groups, and the number of beneficial bacteria, and was increased. It suggests that the FRe potently ameliorate UC as a functional food.
溃疡性结肠炎（UC）是一种慢性炎症性肠病（IBD），其特征为肠道黏膜溃疡。越来越多的证据表明，对共生微生物群的免疫反应失调与IBD的发病机制有关。先前的研究已证明，通过与[具体菌种1]和[具体菌种2]进行三重发酵得到的发酵米提取物（FRe）具有有益的益生菌作用。因此，研究了FRe对UC的治疗潜力。将葡聚糖硫酸钠诱导的UC小鼠模型分别口服蒸馏水作为对照、柳氮磺胺吡啶或300、200和100mg/kg的FRe，每天一次，持续一周。UC对照组出现体重减轻、血便和结肠缩短。然而，FRe，尤其是300mg/kg剂量的FRe，导致体重减轻、疾病活动指数评分和结肠重量降低，以及结肠直肠长度增加。组织病理学分析显示，FRe组的结肠隐窝和黏膜损伤有轻度变化，同时炎症受到抑制。事实上，FRe减少了中性粒细胞浸润和促炎细胞因子（即肿瘤坏死因子-α、白细胞介素-6/-8）的产生。这伴随着核因子-κB的下调。FRe组中负责肠道屏障完整性的基因表达（即[相关基因1]/[相关基因2]、[相关基因3]、[相关基因4]/[相关基因5]）上调。此外，FRe减少了脂质过氧化并增强了抗氧化活性。有趣的是，FRe组的微生物群失调得到缓解，有益细菌[有益菌1]和[有益菌2]的数量增加。这表明FRe作为一种功能性食品可有效改善UC。
