原花青素稳定的金纳米粒子的绿色合成及其作为潜在抗糖尿病和抗氧化剂的研究
Green Synthesis of Gold Nanoparticles Capped with Procyanidins from as Potential Antidiabetic and Antioxidant Agents.
机构信息
Chemistry Department, Cape Peninsula University of Technology, Symphony Rd., Bellville 7535, South Africa.
Electron Microscope Unit, University of the Western Cape, Bellville 7535, South Africa.
出版信息
Biomolecules. 2020 Mar 13;10(3):452. doi: 10.3390/biom10030452.
In this study, procyanidins fractions of dimers and trimers (F1-F2) from the total extract (LSTE) were investigated for their chemical constituents. The total extract and the procyanidins were employed in the synthesis of gold nanoparticles (Au NPs) and fully characterized. Au NPs of 6, 24 and 21 nm were obtained using LSTE, F1 and F2 respectively. Zeta potential and in vitro stability studies confirmed the stability of the particles. The enzymatic activity of LSTE, F1, F2 and their corresponding Au NPs showed strong inhibitory alpha-amylase activity where F1 Au NPs demonstrated the highest with IC of 1.88 µg/mL. On the other hand, F2 Au NPs displayed the strongest alpha-glucosidase activity at 4.5 µg/mL. F2 and F2 Au NPs also demonstrated the highest antioxidant activity, 1834.0 ± 4.7 μM AAE/g and 1521.9 ± 3.0 μM TE/g respectively. The study revealed not only the ability of procyanidins dimers (F1 and F2) in forming biostable and bioactive Au NPs but also, a significant enhancement of the natural products activities, which could improve the smart delivery in future biomedical applications.
在这项研究中，我们研究了来自总提取物（LSTE）的二聚体和三聚体（F1-F2）原花青素的化学成分。总提取物和原花青素被用于合成金纳米粒子（Au NPs），并进行了全面的表征。使用 LSTE、F1 和 F2 分别获得了 6、24 和 21nm 的 Au NPs。Zeta 电位和体外稳定性研究证实了颗粒的稳定性。LSTE、F1、F2 及其相应的 Au NPs 的酶活性显示出强烈的抑制α-淀粉酶活性，其中 F1 Au NPs 的 IC 为 1.88μg/mL。另一方面，F2 Au NPs 在 4.5μg/mL 时显示出最强的α-葡萄糖苷酶活性。F2 和 F2 Au NPs 还表现出最高的抗氧化活性，分别为 1834.0±4.7μM AAE/g 和 1521.9±3.0μM TE/g。该研究不仅揭示了二聚体原花青素（F1 和 F2）形成生物稳定和生物活性 Au NPs 的能力，而且还显著增强了天然产物的活性，这可能有助于未来生物医学应用中的智能输送。
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