Tamakoshi Keigo, Kondo Haruna, Akatsuka Naho, Meguro Kota, Takahashi Yuri
Department of Physical Therapy, Niigata University of Health and Welfare, Niigata, Japan.
Uonuma Kikan Hospital, Niigata, Japan.
J Stroke Cerebrovasc Dis. 2020 May;29(5):104668. doi: 10.1016/j.jstrokecerebrovasdis.2020.104668. Epub 2020 Mar 14.
The purpose of this study was to evaluate the natural recovery process and tissue injury associated with cerebral hemorrhage and cerebral infarction, which were induced to the same degree, in the striatum of rats.
Male Wistar rats were divided into intracerebral hemorrhagic (ICH) and ischemia (ISC) groups, with the ICH group injected with a collagenase solution and the ISC group injected with an endothelin-1 solution. In the SHAM group, physiological saline was injected. Motor function was evaluated by the ladder and forelimb placing tests on the first day before surgery and the first, seventh, and 14th day after surgery. On day 15 after surgery, brain tissue was harvested and frozen sections were prepared. Nissl staining was performed, and the tissue loss, ventricular, and hemispheric volumes were analyzed.
On the first day of surgery, the ICH group had significantly decreased motor function compared with the ISC group. However, subsequent recovery of motor function was faster in the ICH group than that in the ISC group. In addition, tissue loss and hemispheric volumes were significantly higher in the ISC group than those in the ICH group, whereas the ventricular volume was significantly higher in the ICH group than that in the ISC group.
Collectively, our findings indicate that, in ICH and ISC where the brain damage involves the same site and is approximately the same size, motor function is recovered faster in ICH than that in ISC. As such, differences in secondary degeneration are likely affected.
本研究的目的是评估大鼠纹状体内同等程度的脑出血和脑梗死的自然恢复过程及相关组织损伤。
将雄性Wistar大鼠分为脑出血（ICH）组和缺血（ISC）组，ICH组注射胶原酶溶液，ISC组注射内皮素-1溶液。假手术（SHAM）组注射生理盐水。在手术前第1天以及手术后第1、7和14天，通过阶梯试验和前肢放置试验评估运动功能。在手术后第15天，采集脑组织并制备冰冻切片。进行尼氏染色，并分析组织损失、脑室和半球体积。
在手术第一天，ICH组的运动功能与ISC组相比显著下降。然而，ICH组随后的运动功能恢复比ISC组更快。此外，ISC组的组织损失和半球体积显著高于ICH组，而ICH组的脑室体积显著高于ISC组。
总体而言，我们的研究结果表明，在脑损伤部位相同且大小相近的ICH和ISC中，ICH的运动功能恢复比ISC更快。因此，继发性变性的差异可能受到影响。
