Khurm Muhammad, Chaudhry Bashir A, Uzair Muhammad, Janbaz Khalid H
Faculty of Pharmacy, Natural Product Chemistry Unit, Bahauddin Zakariya University, Multan 60800, Pakistan.
Akson College of Pharmacy, Mirpur University of Science and Technology, Mirpur 10250, Pakistan.
Medicines (Basel). 2016 Jul 28;3(3):20. doi: 10.3390/medicines3030020.
Willd. (Chitiphal) is a medicinally important herb that belongs to the family. Traditionally, this plant was used in the medication therapy of various ailments in different populations of the world. The aim of the study is to probe the therapeutic aspects of described in the traditional folklore history of medicines. In the present study, the dichloromethane crude extract of this plant was screened to explore the antimicrobial, cytotoxic, phytotoxic and antioxidant potential of . For antibacterial, antifungal and antioxidant activities, microplate alamar blue assay (MABA), agar tube dilution method and diphenyl picryl hydrazine (DPPH) radical-scavenging assay were used, respectively. The cytotoxic and phytotoxic potential were demonstrated by using brine shrimp lethality bioassay and assay. The crude extract displayed positive cytotoxic activity in the brine shrimp lethality assay, with 23 of 30 shrimps dying at the concentration of 1000 µg/mL. It also showed moderate phytotoxic potential with percent inhibition of 50% at the concentration of 1000 µg/mL. The crude extract exhibited no significant antibacterial activity against , , and . Non-significant antifungal and radical scavenging activity was also shown by the dichloromethane crude extract. It is recommended that scientists focus on the identification and isolation of beneficial bioactive constituents with the help of advanced scientific methodologies that seems to be helpful in the synthesis of new therapeutic agents of desired interest.
威尔德（奇蒂法尔）是一种具有重要药用价值的草本植物，属于该科。传统上，这种植物在世界不同人群的各种疾病药物治疗中被使用。本研究的目的是探究传统民间医学历史中所描述的该植物的治疗作用。在本研究中，对这种植物的二氯甲烷粗提物进行筛选，以探索其抗菌、细胞毒性、植物毒性和抗氧化潜力。对于抗菌、抗真菌和抗氧化活性，分别使用了微孔板alamar蓝分析法（MABA）、琼脂试管稀释法和二苯基苦味酰基肼（DPPH）自由基清除分析法。通过卤虫致死生物测定法和……测定法证明了其细胞毒性和植物毒性潜力。粗提物在卤虫致死测定中显示出阳性细胞毒性活性，在1000μg/mL浓度下，30只卤虫中有23只死亡。它在1000μg/mL浓度下也显示出中等的植物毒性潜力，抑制率为50%。粗提物对……、……、……和……没有显著的抗菌活性。二氯甲烷粗提物也显示出不显著的抗真菌和自由基清除活性。建议科学家借助先进的科学方法专注于有益生物活性成分的鉴定和分离，这似乎有助于合成所需的新型治疗剂。
