Department of Plant Sciences, College of Agriculture and Bio-resources, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada.
AgriGenome Labs Pvt. Ltd., Hyderabad 500 078, India.
Int J Mol Sci. 2020 Mar 17;21(6):2043. doi: 10.3390/ijms21062043.
Environmental stress hampers pea productivity. To understand the genetic basis of heat resistance, a genome-wide association study (GWAS) was conducted on six stress responsive traits of physiological and agronomic importance in pea, with an objective to identify the genetic loci associated with these traits. One hundred and thirty-five genetically diverse pea accessions from major pea growing areas of the world were phenotyped in field trials across five environments, under generally ambient (control) and heat stress conditions. Statistical analysis of phenotype indicated significant effects of genotype (G), environment (E), and G × E interaction for all traits. A total of 16,877 known high-quality SNPs were used for association analysis to determine marker-trait associations (MTA). We identified 32 MTAs that were consistent in at least three environments for association with the traits of stress resistance: six for chlorophyll concentration measured by a soil plant analysis development meter; two each for photochemical reflectance index and canopy temperature; seven for reproductive stem length; six for internode length; and nine for pod number. Forty-eight candidate genes were identified within 15 kb distance of these markers. The identified markers and candidate genes have potential for marker-assisted selection towards the development of heat resistant pea cultivars.
环境胁迫会阻碍豌豆的产量。为了了解耐热性的遗传基础，对豌豆的 6 个生理和农艺重要的应激响应性状进行了全基因组关联研究（GWAS），目的是鉴定与这些性状相关的遗传位点。来自世界主要豌豆种植区的 135 个遗传多样性豌豆品系在五个环境下的田间试验中进行了表型分析，包括一般环境（对照）和热胁迫条件。表型的统计分析表明，所有性状的基因型（G）、环境（E）和 G×E 互作都有显著影响。共使用了 16877 个已知的高质量 SNP 进行关联分析，以确定标记-性状关联（MTA）。我们确定了 32 个在至少三个环境中与抗胁迫性状相关的 MTAs：6 个与土壤植物分析开发计测量的叶绿素浓度相关；光合作用反射指数和冠层温度各 2 个；生殖茎长 7 个；节间长度 6 个；荚数 9 个。在这些标记物 15kb 距离内鉴定出 48 个候选基因。鉴定出的标记和候选基因有可能用于耐热豌豆品种的分子辅助选择。
