Population Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
Department of Livestock Services, Farmgate, Dhaka, 1215, Bangladesh.
Sci Rep. 2022 Jun 25;12(1):10818. doi: 10.1038/s41598-022-14883-7.
Antimicrobial resistance (AMR) among foodborne bacteria is a well-known public health problem. A sink survey was conducted to determine the AMR pattern of common foodborne bacteria in cloacal swab of broiler chickens and sewage samples from five wholesale chicken markets of Dhaka city in Bangladesh. Bacteria were identified by culture-based and molecular methods, and subjected to antimicrobial susceptibility testing. Resistance genes were identified by multiplex PCR and sequencing. Multidrug resistance (MDR) was observed in 93.2% of E. coli, 100% of Salmonella spp., and 97.2% of S. aureus from cloacal swab samples. For sewage samples, 80% of E. coli, and 100% of Salmonella and S. aureus showed MDR. Noteworthy, 8.3% of S. aureus from cloacal swab samples showed possible extensively drug resistance. Antimicrobial resistance genes (beta-lactamase-blaTEM, blaSHV; quinolone resistance gene-qnrS) were detected in a number of E. coli and Salmonella isolates from cloacal swab and sewage samples. The methicillin resistance gene (mecA) was detected in 47.2% and 25% S. aureus from cloacal swab and sewage samples, respectively. The findings envisage the potential public health risk and environmental health hazard through spillover of common foodborne MDR bacteria.
食源性病原体的耐药性（AMR）是一个众所周知的公共卫生问题。本研究采用基于培养和分子的方法进行了一项汇水点调查，以确定孟加拉国达卡市五个批发鸡肉市场中肉鸡泄殖腔拭子和污水样本中常见食源性病原体的耐药模式。对分离出的细菌进行了药敏试验，并通过多重 PCR 和测序鉴定了耐药基因。在泄殖腔拭子样本中，93.2%的大肠杆菌、100%的沙门氏菌和 97.2%的金黄色葡萄球菌表现出多药耐药性（MDR）。对于污水样本，80%的大肠杆菌、100%的沙门氏菌和金黄色葡萄球菌表现出 MDR。值得注意的是，8.3%的金黄色葡萄球菌可能表现出广泛耐药性。在泄殖腔拭子和污水样本中，许多大肠杆菌和沙门氏菌分离株中检测到了耐药基因（β-内酰胺酶 blaTEM、blaSHV；喹诺酮耐药基因 qnrS）。在泄殖腔拭子和污水样本中，47.2%和 25%的金黄色葡萄球菌分别携带耐甲氧西林基因（mecA）。这些发现表明，常见食源性病原体的 MDR 细菌通过溢出可能对公共卫生和环境卫生造成风险和危害。
