Department of Materials Science and Engineering, UNC/NCSU Joint Department of Biomedical Engineering, NC State University, Raleigh, NC 27695, USA.
Identify Sensors Biologics, 1203 W. State St., West Lafayette, IN 47907, USA.
Biosensors (Basel). 2022 Mar 27;12(4):198. doi: 10.3390/bios12040198.
Opioid drugs are extremely potent synthetic analytes, and their abuse is common around the world. Hence, a rapid and point-of-need device is necessary to assess the presence of this compound in body fluid so that a timely countermeasure can be provided to the exposed individuals. Herein, we present an attractive microneedle sensing platform for the detection of the opioid drug fentanyl in real serum samples using an electrochemical detection method. The device contained an array of pyramidal microneedle structures that were integrated with platinum (Pt) and silver (Ag) wires, each with a microcavity opening. The working sensor was modified by graphene ink and subsequently with 4 (3-Butyl-1-imidazolio)-1-butanesulfonate) ionic liquid. The microneedle sensor showed direct oxidation of fentanyl in liquid samples with a detection limit of 27.8 μM by employing a highly sensitive square-wave voltammetry technique. The resulting microneedle-based sensing platform displayed an interference-free fentanyl detection in diluted serum without conceding its sensitivity, stability, and response time. The obtained results revealed that the microneedle sensor holds considerable promise for point-of-need fentanyl detection and opens additional opportunities for detecting substances of abuse in emergencies.
阿片类药物是非常有效的合成分析物，其滥用在全球范围内很常见。因此，有必要快速且在需要时的设备来评估体液中是否存在这种化合物，以便及时为暴露的个体提供对策。在这里，我们提出了一种有吸引力的微针传感平台，用于使用电化学检测方法检测真实血清样本中的阿片类药物芬太尼。该装置包含一个阵列的金字塔形微针结构，与铂（Pt）和银（Ag）线集成在一起，每个线都有一个微腔开口。工作传感器通过石墨烯墨水进行修饰，随后用 4（3-丁基-1-咪唑啉）-1-丁烷磺酸）离子液体。微针传感器通过采用高灵敏度的方波伏安法技术，在液体样品中直接氧化芬太尼，检测限为 27.8 μM。基于微针的传感平台在稀释的血清中进行无干扰的芬太尼检测，同时保持其灵敏度、稳定性和响应时间。所得结果表明，微针传感器在即时芬太尼检测方面具有很大的潜力，并为紧急情况下检测滥用物质开辟了更多机会。
