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泰国“Mahat”(桑科)物种复合体的分类学笔记

Taxonomic Notes on the 'Mahat' ( and , Moraceae) Species Complex in Thailand.

作者信息

Aneklaphakij Chaiwat, Bunsupa Somnuk, Sirichamorn Yotsawate, Bongcheewin Bhanubong, Satitpatipan Veena

机构信息

Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.

Department of Biology, Faculty of Science, Silpakorn University, Sanam Chandra Palace Campus, Nakhon Pathom 73000, Thailand.

出版信息

Plants (Basel). 2020 Mar 22;9(3):391. doi: 10.3390/plants9030391.

Abstract

'Mahat' is a well-known medicinal plant utilized in Thailand. The Thai name 'Mahat' has been used in many scientific articles for years. However, it is, unpredictably, a homonym of two scientific names in Flora of Thailand, i.e., and . Additionally, both species are complex due to their high morphological variation. This causes difficulties in species identification especially when this Thai name is referred to as the scientific name for research publication, quality control of pharmaceutical raw materials, and registration of pharmaceutical products. In this study, we scrutinized the taxonomy of 'Mahat' by detailed examination of its morphology and distribution, including molecular and qualitative phytochemical studies. Leaf surfaces were inspected using scanning electron microscopy. The phylogeny of both species was studied using DNA sequences of nuclear and plastid regions. Chromatographic fingerprints, focusing on the major active compound oxyresveratrol, were identified using high-performance liquid chromatography. According to our current study, phylogenetic evidence showed that some samples of both species were clustered together in the same clade and phytochemical fingerprints were almost identical. These results are valuable data for taxonomic revision in the near future and reveal the possible utilization of as a new material source of oxyresveratrol in the pharmaceutical industry.

摘要

“Mahat”是泰国一种著名的药用植物。泰国名字“Mahat”多年来一直被用于许多科学文章中。然而,不可预测的是,它是《泰国植物志》中两个学名的同音异义词,即[此处缺失两个学名]。此外,这两个物种都因其高度的形态变异而很复杂。这给物种鉴定带来了困难,尤其是当这个泰国名字被用作研究出版物的学名、药用原材料的质量控制以及药品注册时。在本研究中,我们通过详细检查其形态和分布,包括分子和定性植物化学研究,仔细审查了“Mahat”的分类学。使用扫描电子显微镜检查叶片表面。利用核区和质体区的DNA序列研究了这两个物种的系统发育。使用高效液相色谱法鉴定了聚焦于主要活性化合物氧化白藜芦醇的色谱指纹图谱。根据我们目前的研究,系统发育证据表明这两个物种的一些样本聚集在同一个分支中,并且植物化学指纹图谱几乎相同。这些结果是近期分类学修订的宝贵数据,并揭示了[此处缺失相关物种]作为制药行业氧化白藜芦醇新原料来源的可能用途。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3936/7154811/e651db58190d/plants-09-00391-g001.jpg

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