壳聚糖/胶原蛋白膜的结构-性能关系及其在生物医学中的应用潜力。

Structure-properties relationship of chitosan/collagen films with potential for biomedical applications.

机构信息

BIOMAT Research Group, University of the Basque Country (UPV/EHU), Escuela de Ingeniería de Gipuzkoa, Plaza de Europa 1, 20018, Donostia-San Sebastián, Spain.

NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006, Vitoria-Gasteiz, Spain.

出版信息

Carbohydr Polym. 2020 Jun 1;237:116159. doi: 10.1016/j.carbpol.2020.116159. Epub 2020 Mar 16.

DOI:10.1016/j.carbpol.2020.116159

PMID:32241409

Abstract

Chitosan/collagen films were developed and characterized in order to assess the suitability of these films for biomedical applications. Hence, physicochemical, thermal, barrier and mechanical properties were analyzed and related to the film structure, which showed the prevalence of the triple helix of native collagen after the addition of chitosan. Furthermore, collagen fiber diameter changed from 3.9 ± 0.6 μm, for collagen films without chitosan, to 1.8 ± 0.5 μm, for collagen films with low molecular weight chitosan. These results suggested interactions between collagen and chitosan molecules, as observed by Fourier transform infrared (FTIR) analysis. Regarding film barrier properties, chitosan/collagen films showed a water vapor transmission rate around 1174 g m day, suitable for biomedical applications such as wound healing. Additionally, biological tests confirmed that the chitosan/collagen films developed are suitable for biomedical applications.

摘要

为了评估这些薄膜在生物医学应用中的适用性，开发并表征了壳聚糖/胶原薄膜。因此，分析了物理化学、热学、阻隔和机械性能，并将其与薄膜结构相关联，结果表明壳聚糖的加入促进了天然胶原三螺旋结构的形成。此外，胶原纤维直径从不含壳聚糖的胶原薄膜的 3.9±0.6μm 变为低分子量壳聚糖的胶原薄膜的 1.8±0.5μm。这些结果表明，如傅里叶变换红外（FTIR）分析所示，胶原和壳聚糖分子之间存在相互作用。关于薄膜阻隔性能，壳聚糖/胶原薄膜的水蒸气透过率约为 1174g m day，适用于伤口愈合等生物医学应用。此外，生物学测试证实，所开发的壳聚糖/胶原薄膜适用于生物医学应用。