Department of Pathology and Laboratory Medicine, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA; Center for Substance Abuse Research, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
Department of Pathology and Laboratory Medicine, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
Brain Behav Immun. 2020 Aug;88:363-380. doi: 10.1016/j.bbi.2020.03.034. Epub 2020 Mar 31.
Electronic cigarette (e-cigarette) use has grown substantially since inception, particularly among adolescents and combustible tobacco users. Several cigarette smoke constituents with known neurovascular effect are present in e-cigarette liquids or formed during the vapor generation. The present study establishes inhaled models of cigarette and e-cigarette use with normalized nicotine delivery, then characterizes the impact on blood-brain barrier (BBB) function. Sequencing of microvessel RNA following exposure revealed downregulation of several genes with critical roles in BBB function. Reduced protein expression of Occludin and Glut1 is also observed at the tight junction in all groups following exposure. Pro-inflammatory changes in leukocyte-endothelial cell interaction are also noted, and mice exposed to nicotine-free e-cigarettes have impaired novel object recognition performance. On this basis, it is concluded that long term e-cigarette use may adversely impact neurovascular health. The observed effects are noted to be partly independent of nicotine content and nicotine may even serve to moderate the effects of non-nicotinic components on the blood-brain barrier.
电子烟（e-cigarette）自问世以来使用量大幅增长，尤其是在青少年和可燃烟草使用者中。电子烟液体中存在几种已知具有神经血管作用的香烟烟雾成分，或在蒸气生成过程中形成。本研究建立了具有标准化尼古丁输送的香烟和电子烟使用的吸入模型，然后描述了对血脑屏障（BBB）功能的影响。暴露后对微血管 RNA 进行测序显示，几个在 BBB 功能中具有关键作用的基因表达下调。暴露后所有组的紧密连接处也观察到 Occludin 和 Glut1 的蛋白表达减少。白细胞-内皮细胞相互作用中的促炎变化也被注意到，并且暴露于无尼古丁电子烟的小鼠的新物体识别性能受损。基于此，可以得出结论，长期使用电子烟可能会对神经血管健康产生不利影响。观察到的影响部分独立于尼古丁含量，并且尼古丁甚至可能有助于调节非尼古丁成分对血脑屏障的影响。
