Nozza Emma, Melzi Gloria, Marabini Laura, Marinovich Marina, Piazza Stefano, Khalilpour Saba, Dell'Agli Mario, Sangiovanni Enrico
Department of Pharmacological and Biomolecular Sciences (DiSFeB), Università degli Studi di Milano, 20133 Milan, Italy.
Department of Environmental Science and Policy (ESP), Università degli Studi di Milano, 20133 Milan, Italy.
Antioxidants (Basel). 2020 Apr 1;9(4):292. doi: 10.3390/antiox9040292.
L. (sumac) is a small plant widely diffused in the Mediterranean region. Its fruit are often consumed as a spice but are also present in traditional medicine of several countries. Recently, interest in this plant has increased and many scientific works reported its beneficial effects including antioxidant and anti-inflammatory properties. Plant extracts can be successfully used against ultraviolet rays, which are able to reach and damage the human skin; however, sumac extracts were never applied to this usage. Thus, in this study, we used a macerated ethanol extract of L. dried fruit (mERC) to demonstrate its preventive role against the damage induced by ultraviolet-A rays (UV-A) on microvascular endothelial cells (HMEC-1). In vitro effects of the extract pre-treatment and UV-A exposure were evaluated in detail. The antioxidant capacity was assessed by reactive oxygen species (ROS) formation and cellular antioxidant activity measurement. Genoprotective effects of mERC were investigated as well. Our findings indicate that the extract acts as a cell cycle inhibitor or apoptosis inducer, according to the level of damage. The present work provides new insights into the usage of extracts against skin injuries.
漆树是一种广泛分布于地中海地区的小植物。其果实常被用作香料，在一些国家的传统医学中也有应用。最近，人们对这种植物的兴趣有所增加，许多科学研究报告了它的有益作用，包括抗氧化和抗炎特性。植物提取物可成功用于抵御能够到达并损伤人体皮肤的紫外线；然而，漆树提取物从未用于此用途。因此，在本研究中，我们使用漆树干果的乙醇浸提物（mERC）来证明其对紫外线A（UV-A）诱导的微血管内皮细胞（HMEC-1）损伤的预防作用。详细评估了提取物预处理和UV-A照射的体外效果。通过活性氧（ROS）形成和细胞抗氧化活性测量来评估抗氧化能力。还研究了mERC的基因保护作用。我们的研究结果表明，根据损伤程度，该提取物可作为细胞周期抑制剂或凋亡诱导剂。本研究为提取物在皮肤损伤方面的应用提供了新的见解。
