Division of Microbial Technology, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226 001, India.
Academy of Scientific and Innovative Research, AcSIR, Ghaziabad, 201002, India.
Sci Rep. 2020 Apr 3;10(1):5855. doi: 10.1038/s41598-020-62725-1.
Phosphorus (P) availability and salinity stress are two major constraints for agriculture productivity. A combination of salinity and P starvation is known to be more deleterious to plant health. Plant growth promoting rhizobacteria are known to ameliorate abiotic stress in plants by increasing the availability of different nutrients. However, interaction mechanisms of plant grown under salinity and P stress condition and effect of beneficial microbe for stress alleviation is still obscure. Earlier we reported the molecular insight of auxin producing, phosphate solubilising Pseudomonas putida MTCC 5279 (RAR) mediated plant growth promotion in Arabidopsis thaliana. In present study new trait of proline and phosphatase production of RAR and its impact on modulation of physiological phenomenon under phosphate starved-salinity stress condition in A. thaliana has been investigated. Different physiological and molecular determinants under RAR- A. thaliana interaction showed that auxin producing RAR shows tryptophan dependence for growth and proline production in ATP dependant manner under salinity stress. However, under P deprived conditions growth and proline production are independent of tryptophan. RAR mediated lateral root branching and root hair density through modulation of abscisic acid signalling was observed. Acidic phosphatase activity under P starved and salinity stress condition was majorly modulated along with ROS metabolism and expression of stress responsive/phosphate transporter genes. A strong correlation of different morpho-physiological factor with RAR + salt conditions, showed We concluded that enhanced adverse effect of salinity with unavailability of P was dampened in presence of P. putida MTCC 5279 (RAR) in A. thaliana, though more efficiently salinity stress conditions. Therefore, alleviation of combined stress of salinity induced phosphate nutrient deficiency by inoculation of beneficial microbe, P. putida MTCC 5279 offer good opportunities for enhancing the agricultural productivity.
磷（P）的可利用性和盐度胁迫是农业生产力的两个主要限制因素。已知盐度和 P 饥饿的组合对植物健康更具危害性。已发现植物促生根际细菌通过增加不同养分的可利用性来缓解植物的非生物胁迫。然而，在盐度和 P 胁迫条件下生长的植物的相互作用机制以及有益微生物对缓解胁迫的影响仍然不清楚。早些时候，我们报道了产生长素、溶磷假单胞菌 MTCC 5279（RAR）介导拟南芥生长促进的分子见解。在本研究中，研究了 RAR 产生脯氨酸和磷酸酶的新特性及其对拟南芥缺磷-盐胁迫条件下生理现象调节的影响。在 RAR-拟南芥相互作用下的不同生理和分子决定因素表明，产生长素的 RAR 在盐胁迫下以依赖三磷酸腺苷的方式依赖色氨酸生长和产生脯氨酸。然而，在缺磷条件下，生长和脯氨酸的产生与色氨酸无关。通过调节脱落酸信号，RAR 介导了侧根分枝和根毛密度。在缺磷和盐胁迫条件下，酸性磷酸酶活性主要与 ROS 代谢和应激响应/磷酸盐转运体基因的表达一起调节。不同形态生理因子与 RAR+盐条件的强烈相关性表明，在拟南芥中，尽管盐度胁迫条件更有效，但在 P. putida MTCC 5279（RAR）存在的情况下，增强了盐度与 P 不可用的不利影响。因此，通过接种有益微生物 P. putida MTCC 5279 缓解盐度诱导的磷营养缺乏的复合胁迫，为提高农业生产力提供了良好的机会。
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