脂肪酸功能化安德森型多金属氧酸盐与人血清白蛋白的结合。
Binding of a Fatty Acid-Functionalized Anderson-Type Polyoxometalate to Human Serum Albumin.
机构信息
Fakultät für Chemie, Institut für Biophysikalische Chemie, Universität Wien, Althanstraße 14, 1090 Wien, Austria.
Fakultät für Chemie, Zentrum für Röntgenstrukturanalyse, Universität Wien, Währinger Straße 42, 1090 Wien, Austria.
出版信息
Inorg Chem. 2020 Apr 20;59(8):5243-5246. doi: 10.1021/acs.inorgchem.9b03407. Epub 2020 Apr 7.
The Anderson-type hexamolybdoaluminate functionalized with lauric acid (LA), (TBA)[Al(OH)MoO{(OCH)CNHCOCH}]·9HO (TBA-AlMo-LA, where TBA = tetrabutylammonium), was prepared via two synthetic routes and characterized by thermogravimetric and elemental analyses, mass spectrometry, IR and H NMR spectroscopy, and powder and single-crystal X-ray diffraction. The interaction of TBA-AlMo-LA with human serum albumin (HSA) was investigated via fluorescence and circular dichroism spectroscopy. The results revealed that TBA-AlMo-LA binds strongly to HSA (63% quenching at an HSA/TBA-AlMo-LA ratio of 1:1), exhibiting static quenching. In contrast to TBA-AlMo-LA, the nonfunctionalized polyoxometalate, Na(HO)[Al(OH)MoO]·2HO (AlMo), showed weak binding toward HSA (22% quenching at a HSA/AlMo ratio of 1:25). HSA binding was confirmed by X-ray structure analysis of the HSA-Myr-AlMo-LA complex (Myr = myristate). These results provide a promising lead for the design of novel polyoxometalate-based hybrids that are able to exploit HSA as a delivery vehicle to improve their pharmacokinetics and bioactivity.
用月桂酸功能化的安德森型六钼铝酸（LA），（TBA）[Al（OH）MoO{（OCH）CNHCOCH}]·9HO（TBA-AlMo-LA，其中 TBA = 四丁基铵），通过两种合成路线制备，并通过热重和元素分析、质谱、IR 和 H NMR 光谱以及粉末和单晶 X 射线衍射进行了表征。通过荧光和圆二色光谱研究了 TBA-AlMo-LA 与人血清白蛋白（HSA）的相互作用。结果表明，TBA-AlMo-LA 与 HSA 结合紧密（HSA/TBA-AlMo-LA 摩尔比为 1:1 时猝灭率为 63%），表现为静态猝灭。与 TBA-AlMo-LA 不同，未功能化的多金属氧酸盐 Na（HO）[Al（OH）MoO]·2HO（AlMo）对 HSA 的结合能力较弱（HSA/AlMo 摩尔比为 1:25 时猝灭率为 22%）。通过 HSA-Myr-AlMo-LA 配合物（Myr = 肉豆蔻酸）的 X 射线结构分析证实了 HSA 的结合。这些结果为设计新型基于多金属氧酸盐的杂化物提供了有希望的线索，这些杂化物能够利用 HSA 作为递送载体来改善其药代动力学和生物活性。
Zotero 插件