Laboratory of Molecular Toxicology, Faculty of Nature and Life Science, University of Mohamed Seddik Benyahia, 18000 Jijel, Algeria.
Laboratory of Phytochemistry and Physical-Chemical and Biological Analyses, Department of Chemistry, Faculty of Exact Sciences, University of Mentouri Constantine, 25000 Constantine, Algeria.
Molecules. 2020 Apr 3;25(7):1647. doi: 10.3390/molecules25071647.
is an Algerian-Tunisian endemic species, which has not been studied yet. Ethyl acetate (EA) and -butanol (Bu) fractions obtained from were investigated for their health benefit properties, in particular with respect to in vivo/in vitro anti-inflammatory and antioxidant activities, as well as their potential to inhibit key enzymes with impact in diabetes (α-glucosidase and α-amylase). The fractions had a distinct phytochemical composition, of which EA was richer in total phenolic compounds (225 mg GAE/g) and mostly composed of the phenylethanoid acetyl martynoside. Compared to EA, Bu had higher amounts of total flavonoids, and according to the result obtained from UHPLC-DAD-ESI-MS analysis, harpagoside (iridoid) was its major phytochemical. EA fraction was quite promising with regard to the in vivo (at 200 mg/kg, po) anti-inflammatory effect (62% and 52% for carrageenan-induced rat paw edema and xylene-induced ear edema tests, respectively), while Bu fraction exhibited a stronger antioxidant capacity in all tests (IC = 68 µg/mL, IC = 18 µg/mL, IC = 18 µg/mL and A = 43 µg/mL for DPPH, ABTS, O scavenging assays and cupric-reducing antioxidant capacity method, respectively). Both fractions also showed a strong effect against α-amylase enzyme (IC = 8 µg/mL and 10 µg/mL for EA and Bu fraction, respectively).
是一种阿尔及利亚-突尼斯地方性物种，尚未进行研究。从 中提取的乙酸乙酯（EA）和 -丁醇（Bu）馏分，因其具有体内/体外抗炎和抗氧化活性，以及抑制对糖尿病有影响的关键酶（α-葡萄糖苷酶和α-淀粉酶）的潜力，而被研究其健康益处特性。这些馏分具有独特的植物化学成分，其中 EA 中总酚类化合物（225 mg GAE/g）含量较高，主要由苯乙醇基乙酰马丁诺苷组成。与 EA 相比，Bu 中总类黄酮的含量更高，根据 UHPLC-DAD-ESI-MS 分析结果，哈巴苷（环烯醚萜）是其主要的植物化学成分。EA 馏分在体内（200 mg/kg，po）抗炎作用方面表现出相当大的潜力（对卡拉胶诱导的大鼠足肿胀和二甲苯诱导的耳肿胀试验的抑制率分别为 62%和 52%），而 Bu 馏分在所有试验中均表现出更强的抗氧化能力（DPPH 的 IC = 68 µg/mL、ABTS 的 IC = 18 µg/mL、O 的 IC = 18 µg/mL 和 Cupric 还原抗氧化能力法的 A = 43 µg/mL）。两个馏分对α-淀粉酶酶也有很强的抑制作用（EA 和 Bu 馏分的 IC 分别为 8 µg/mL 和 10 µg/mL）。
