Fan Ming, Yan Jingxuan, Tan Huaping, Miao Yuting, Hu Xiaohong
School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
J Mater Chem B. 2014 Dec 21;2(47):8399-8405. doi: 10.1039/c4tb01106f. Epub 2014 Oct 28.
Biopolymer-based nanogels have great potential in the field of tissue regenerative medicine. In this work, a magnetic biopolymer nanogel via specific nucleobase pairing was developed for vectoring delivery of cell growth factors. The biopolymer based nanogels chitosan and heparin were established by the Watson-Crick base pairing between thymine and adenine via the hydrogen bonding. The magnetic biopolymer nanogels exhibit quick magnetic responsibility, which were fabricated by encapsulating super-paramagnetic iron oxide nanoparticles. The potential applications of this magnetic nanogel on vectoring delivery of cell growth factors were confirmed by adsorption and release behaviors of bone morphogenetic protein 2 (BMP-2). The existence of heparin made the nanogel achieve a high loading efficiency of BMP-2, and the vectoring delivery of BMP-2 could be easily controlled by the external magnetic field. In vitro cytotoxicity tests demonstrated that incorporation of BMP-2 into this biopolymer nanogel through binding with heparin showed high efficiency to promote MG-63 cells' viabilities, in particular under a magnetic field, which suggested a promising future for cartilage and bone regeneration applications.
基于生物聚合物的纳米凝胶在组织再生医学领域具有巨大潜力。在这项工作中，通过特定的核碱基配对开发了一种磁性生物聚合物纳米凝胶，用于细胞生长因子的载体递送。基于生物聚合物的纳米凝胶壳聚糖和肝素是通过胸腺嘧啶和腺嘌呤之间的沃森-克里克碱基配对经氢键作用形成的。磁性生物聚合物纳米凝胶表现出快速的磁响应性，它是通过包裹超顺磁性氧化铁纳米颗粒制备而成。通过骨形态发生蛋白2（BMP-2）的吸附和释放行为证实了这种磁性纳米凝胶在细胞生长因子载体递送方面的潜在应用。肝素的存在使纳米凝胶实现了BMP-2的高负载效率，并且BMP-2的载体递送可以通过外部磁场轻松控制。体外细胞毒性测试表明，通过与肝素结合将BMP-2掺入这种生物聚合物纳米凝胶中显示出高效促进MG-63细胞活力，特别是在磁场作用下，这表明在软骨和骨再生应用方面具有广阔前景。
