Tsai Kuo-Yang, Lin Hung-Yang, Chen Yi-Wen, Lin Cheng-Yao, Hsu Tuan-Ti, Kao Chia-Tze
Department of Oral and Maxillofacial Surgery, Changhua Christian Hospital, Changhua 500, Taiwan.
Department of Oral and Maxillofacial Surgery, China Medical University Hospital, Taichung 40447, Taiwan.
Materials (Basel). 2017 Jan 13;10(1):65. doi: 10.3390/ma10010065.
In this study, we manufacture and analyze bioactive magnesium-calcium silicate/poly-ε-caprolactone (Mg-CS/PCL) 3D scaffolds for bone tissue engineering. Mg-CS powder was incorporated into PCL, and we fabricated the 3D scaffolds using laser sintering technology. These scaffolds had high porosity and interconnected-design macropores and structures. As compared to pure PCL scaffolds without an Mg-CS powder, the hydrophilic properties and degradation rate are also improved. For scaffolds with more than 20% Mg-CS content, the specimens become completely covered by a dense bone-like apatite layer after soaking in simulated body fluid for 1 day. In vitro analyses were directed using human mesenchymal stem cells (hMSCs) on all scaffolds that were shown to be biocompatible and supported cell adhesion and proliferation. Increased focal adhesion kinase and promoted cell adhesion behavior were observed after an increase in Mg-CS content. In addition, the results indicate that the Mg-CS quantity in the composite is higher than 10%, and the quantity of cells and osteogenesis-related protein of hMSCs is stimulated by the Si ions released from the Mg-CS/PCL scaffolds when compared to PCL scaffolds. Our results proved that 3D Mg-CS/PCL scaffolds with such a specific ionic release and good degradability possessed the ability to promote osteogenetic differentiation of hMSCs, indicating that they might be promising biomaterials with potential for next-generation bone tissue engineering scaffolds.
在本研究中，我们制备并分析了用于骨组织工程的生物活性镁钙硅酸盐/聚ε-己内酯（Mg-CS/PCL）3D支架。将Mg-CS粉末掺入PCL中，并用激光烧结技术制备3D支架。这些支架具有高孔隙率和相互连通设计的大孔及结构。与不含Mg-CS粉末的纯PCL支架相比，其亲水性和降解速率也有所提高。对于Mg-CS含量超过20%的支架，在模拟体液中浸泡1天后，标本完全被致密的骨样磷灰石层覆盖。使用人间充质干细胞（hMSCs）对所有支架进行体外分析，结果表明这些支架具有生物相容性并支持细胞黏附和增殖。随着Mg-CS含量的增加，观察到黏着斑激酶增加并促进了细胞黏附行为。此外，结果表明复合材料中Mg-CS的含量高于10%，与PCL支架相比，Mg-CS/PCL支架释放的Si离子刺激了hMSCs的细胞数量和成骨相关蛋白。我们的结果证明，具有这种特定离子释放和良好降解性的3D Mg-CS/PCL支架具有促进hMSCs成骨分化的能力，表明它们可能是下一代骨组织工程支架中有潜力的生物材料。
