Ghorbani Farnaz, Kim Minjoo, Monavari Mahshid, Ghalandari Behafarid, Boccaccini Aldo R
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Front Bioeng Biotechnol. 2022 Aug 8;10:940070. doi: 10.3389/fbioe.2022.940070. eCollection 2022.
This study utilized extrusion-based 3D printing technology to fabricate calcium-cross-linked alginate dialdehyde-gelatin scaffolds for bone regeneration. The surface of polymeric constructs was modified with mussel-derived polydopamine (PDA) in order to induce biomineralization, increase hydrophilicity, and enhance cell interactions. Microscopic observations revealed that the PDA layer homogeneously coated the surface and did not appear to induce any distinct change in the microstructure of the scaffolds. The PDA-functionalized scaffolds were more mechanically stable (compression strength of 0.69 ± 0.02 MPa) and hydrophilic (contact angle of 26) than non-modified scaffolds. PDA-decorated ADA-GEL scaffolds demonstrated greater durability. As result of the 18-days immersion in simulated body fluid solution, the PDA-coated scaffolds showed satisfactory biomineralization. Based on theoretical energy analysis, it was shown that the scaffolds coated with PDA interact spontaneously with osteocalcin and osteomodulin (binding energy values of -35.95 kJ mol and -46.39 kJ mol, respectively), resulting in the formation of a protein layer on the surface, suggesting applications in bone repair. PDA-coated ADA-GEL scaffolds are capable of supporting osteosarcoma MG-63 cell adhesion, viability (140.18% after 7 days), and proliferation. In addition to increased alkaline phosphatase secretion, osteoimage intensity also increased, indicating that the scaffolds could potentially induce bone regeneration. As a consequence, the present results confirm that 3D printed PDA-coated scaffolds constitute an intriguing novel approach for bone tissue engineering.
本研究利用基于挤出的3D打印技术制备了用于骨再生的钙交联藻酸盐二醛-明胶支架。用贻贝来源的聚多巴胺（PDA）对聚合物构建体的表面进行修饰，以诱导生物矿化、增加亲水性并增强细胞相互作用。显微镜观察表明，PDA层均匀地覆盖在表面，并且似乎没有引起支架微观结构的任何明显变化。与未修饰的支架相比，PDA功能化的支架在机械性能上更稳定（抗压强度为0.69±0.02MPa）且亲水性更强（接触角为26°）。PDA修饰的ADA-GEL支架表现出更高的耐久性。在模拟体液溶液中浸泡18天后，PDA涂层支架显示出令人满意的生物矿化。基于理论能量分析，结果表明，涂有PDA的支架与骨钙素和骨调节素自发相互作用（结合能值分别为-35.95kJ/mol和-46.39kJ/mol），从而在表面形成蛋白质层，这表明其在骨修复中的应用潜力。PDA涂层的ADA-GEL支架能够支持骨肉瘤MG-63细胞的粘附、活力（7天后为140.18%）和增殖。除了碱性磷酸酶分泌增加外，骨影像强度也增加，表明该支架可能诱导骨再生。因此，本研究结果证实，3D打印的PDA涂层支架是骨组织工程中一种引人关注的新方法。
