Wang Jie, Qian Shi, Liu Xuanyong, Xu Lianyi, Miao Xinchao, Xu Zhenyao, Cao Lingyan, Wang Honglin, Jiang Xinquan
Department of Prosthodontics, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China.
J Mater Chem B. 2017 May 14;5(18):3364-3376. doi: 10.1039/c6tb03364d. Epub 2017 Apr 19.
The monocyte/macrophage system plays an essential role in the host response and the fate of endosseous implanted materials. Macrophage behavior was thought to be regulated by nanostructured titanium which has been considered as a very promising candidate for dental implants. However, there is little known for subsequent effects of these activated macrophages on osteogenesis and angiogenesis which were essential for bone integration. Here we presented two different dimensions of titanium nanotubes generated by anodic oxidation at 10 V (NT 10) and 20 V (NT 20), respectively. The behavior of macrophages on the surfaces was evaluated, and their conditioned medium (CM) was collected to stimulate MC3T3 and HUVECs, with commercially pure titanium (cp Ti) as control. We found that NT 20 induced macrophage activation similar to the anti-inflammatory M2 macrophage state with the enhanced expression of IL-10 and ARG, while NT 10 was associated with M1 macrophage phenotype characterized by high levels of IL-1β, iNOS and TNF-α. Furthermore, the osteogenic capacity of MC3T3 in CM from NT 20 was enhanced (NT 20 > NT 10 ≈ cp Ti) and the tube formation capacity of HUVECs was promoted in CM from nanotubular surfaces with increasing tube dimensions (NT 20 > NT 10 > cp Ti). Our data suggest that dental implants with the large nanotube dimension surface could result in a favorable osteoimmunomodulatory microenvironment for the establishment of osseointegration.
单核细胞/巨噬细胞系统在宿主反应和骨内植入材料的转归中起着至关重要的作用。巨噬细胞行为被认为受纳米结构钛的调控，纳米结构钛被视为牙种植体非常有前景的候选材料。然而，关于这些活化巨噬细胞对骨整合所必需的骨生成和血管生成的后续影响却知之甚少。在此，我们展示了分别通过在10 V（NT 10）和20 V（NT 20）下进行阳极氧化生成的两种不同尺寸的钛纳米管。评估了巨噬细胞在其表面的行为，并收集其条件培养基（CM）以刺激MC3T3和人脐静脉内皮细胞（HUVECs），以商业纯钛（cp Ti）作为对照。我们发现，NT 20诱导巨噬细胞活化，类似于抗炎性M2巨噬细胞状态，伴随着白细胞介素-10（IL-10）和精氨酸酶（ARG）表达增强，而NT 10与以高水平白细胞介素-1β（IL-1β）、诱导型一氧化氮合酶（iNOS）和肿瘤坏死因子-α（TNF-α）为特征的M1巨噬细胞表型相关。此外，来自NT 20的CM中MC3T3的成骨能力增强（NT 20 > NT 10 ≈ cp Ti），并且随着管尺寸增加，纳米管表面CM中HUVECs的管形成能力得到促进（NT 20 > NT 10 > cp Ti）。我们的数据表明，具有大尺寸纳米管表面的牙种植体可为骨整合的建立带来有利的骨免疫调节微环境。
