Cai Liang, Pan Yongkang, Tang Songchao, Li Quan, Tang Tingting, Zheng Kai, Boccaccini A R, Wei Shicheng, Wei Jie, Su Jiacan
Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China.
J Mater Chem B. 2017 Nov 14;5(42):8337-8352. doi: 10.1039/c7tb02344h. Epub 2017 Oct 13.
Bone implants of polyetheretherketone (PEEK) have become increasingly popular in the orthopedic field but its bioinertness and poor osteogenic properties significantly limit its applications for bone regeneration and osseointegration with host bone. In this study, by incorporation of mesoporous diopside (MD) into the PEEK matrix, macro-mesoporous PEEK/MD (PM) composites as bone implants with interconnected macropores of 300-400 μm were fabricated using the method of cold press-sintering and salt-leaching. The results showed that the compressive strength, porosity and water absorption of the porous PM composites significantly increased with an increase in the MD content. In addition, the incorporation of MD into PEEK obviously enhanced the mineralization ability of the PM composites, indicating good bioactivity. Moreover, the in vitro cell experiments indicated that the macro-mesoporous PM composites significantly promoted the adhesion and proliferation as well as osteogenic differentiation of MC3T3-E1 cells, which depended on the MD content. The results of synchrotron radiation-based micro-computed tomography (SRmCT) and histological analysis revealed that the porous PM composites possessed excellent osteogenic properties in vivo, which were obviously enhanced with an increase in the MD content. Moreover, the immunohistochemistry evaluation of type I collagen (COL I) and vascular endothelial growth factor (VEGF) confirmed that addition of MD into PEEK obviously enhanced the osteogenesis and vascularization potential of the macro-mesoporous PM composites in vivo. The results suggested that the PM composites containing macropores and mesopores as bone implants had great potential for bone repair/substitution.
聚醚醚酮（PEEK）骨植入物在骨科领域越来越受欢迎，但其生物惰性和成骨性能差显著限制了其在骨再生和与宿主骨骨整合方面的应用。在本研究中，通过将介孔透辉石（MD）掺入PEEK基体中，采用冷压烧结和盐浸法制备了具有300 - 400μm相互连通大孔的大孔-介孔PEEK/MD（PM）复合骨植入物。结果表明，多孔PM复合材料的抗压强度、孔隙率和吸水率随MD含量的增加而显著提高。此外，将MD掺入PEEK中明显增强了PM复合材料的矿化能力，表明其具有良好的生物活性。而且，体外细胞实验表明，大孔-介孔PM复合材料显著促进了MC3T3-E1细胞的黏附、增殖以及成骨分化，这取决于MD含量。基于同步辐射的微计算机断层扫描（SRmCT）和组织学分析结果显示，多孔PM复合材料在体内具有优异的成骨性能，且随着MD含量的增加明显增强。此外，对I型胶原（COL I）和血管内皮生长因子（VEGF）的免疫组织化学评估证实，将MD添加到PEEK中明显增强了大孔-介孔PM复合材料在体内的成骨和血管生成潜力。结果表明，含有大孔和介孔的PM复合材料作为骨植入物在骨修复/替代方面具有巨大潜力。
