Reidy M A, Bowyer D E
Atherosclerosis. 1977 Mar;26(3):319-28. doi: 10.1016/0021-9150(77)90084-3.
A single injection of endotoxin P45 Poly Serratia marcescens was used to induce endothelial injury in rabbits. The aortic endothelium was examined by Scanning Electron Microscopy (SEM), at various times after administration of endotoxin, using the technique of silver staining and pressure fixation. Within one hour after injection, some endothelial cells were curled-up and spindle-shaped in appearance. Areas of aorta devoid of endothelial cover were occasionally observed and platelets were sometimes found adhering to these sites. Two and four weeks after initial injury no spindle-shaped cells were found. Instead, some endothelial cells were heavily stained with silver. Small denuded zones were still found and these were surrounded by brightly silver-stained cells. This study confirms that endotoxin rapidly causes endothelial injury and suggests that regenerating endothelial cells which were formed following injury are avidly stained by silver salts and appear as bright cells by SEM.
单次注射内毒素P45多粘类芽孢杆菌以诱导兔内皮损伤。在内毒素给药后的不同时间，采用银染和压力固定技术，通过扫描电子显微镜（SEM）检查主动脉内皮。注射后一小时内，一些内皮细胞卷曲且呈纺锤形。偶尔观察到主动脉无内皮覆盖的区域，有时还发现血小板附着于这些部位。初次损伤后两周和四周未发现纺锤形细胞。相反，一些内皮细胞被银染得很深。仍发现有小的裸露区域，这些区域被银染明亮的细胞包围。本研究证实内毒素可迅速引起内皮损伤，并表明损伤后形成的再生内皮细胞被银盐强烈染色，在扫描电镜下呈现为明亮的细胞。
