Davies P F, Reidy M A, Goode T B, Bowyer D E
Atherosclerosis. 1976 Oct;25(1):125-30. doi: 10.1016/0021-9150(76)90054-x.
The luminal surface of fatty lesions of atherosclerosis was viewed by scanning electron microscopy (SEM). Endothelial cells were outlined by staining intercellular junctions with silver and the aortas were fixed in situ at physiological pressure. When aortas were dehydrated by passage through organic solvents followed by critical point drying from liquid CO2, there was considerable disruption of the luminal surface and it was not possible to correctly interpret the morphological integrity of the endothelium. In contrast, simple air-drying of aortas, without solvent dehydration after fixation, allowed the integrity of the cell layer overlying the lesion to be evaluated. The success of this technique was attributed to the retention of arterial lipids during dehydration of the tissue.
通过扫描电子显微镜（SEM）观察动脉粥样硬化脂肪病变的管腔表面。用银染细胞间连接勾勒出内皮细胞，主动脉在生理压力下原位固定。当主动脉通过有机溶剂脱水，随后从液态二氧化碳进行临界点干燥时，管腔表面出现相当大的破坏，无法正确解释内皮的形态完整性。相比之下，主动脉简单风干，固定后不进行溶剂脱水，能够评估病变上方细胞层的完整性。该技术的成功归因于组织脱水过程中动脉脂质的保留。
