Yinda Claude Kwe, Rector Annabel, Zeller Mark, Conceição-Neto Nádia, Heylen Elisabeth, Maes Piet, Ghogomu Stephen Mbigha, Van Ranst Marc, Matthijnssens Jelle
KULeuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Viral Metagenomics, Leuven, Belgium.
KULeuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory for Clinical and Epidemiological Virology, Leuven, Belgium.
Virol Rep. 2016 Dec;6:74-80. doi: 10.1016/j.virep.2016.08.001. Epub 2016 Aug 20.
A number of PVs have been described in bats but to the best of our knowledge not from feces. Using a previously described NetoVIR protocol, pooled fecal samples (Eh) were treated and sequenced by Illumina next generation sequencing technology. Two complete genomes of novel PVs (EhPV2 and EhPV3) and 3 partial sequences (BATPV61, BATPV890a and BATPV890b) were obtained and analysis showed that the EhPV2 and EhPV3 major capsid proteins cluster with and share 60-64% nucleotide identity with that of PV1, thus representing new species of PVs within the genus . The other PVs clustered in different branches of our phylogenetic tree and may potentially represent novel species and/or genera. This points to the vast diversity of PVs in bats and in bats in particular, therefore adding support to the current concept that PV evolution is more complex than merely strict PV-host co-evolution.
在蝙蝠中已描述了多种细小病毒，但据我们所知，尚未从粪便中发现过。使用先前描述的NetoVIR方案，对合并的粪便样本（Eh）进行处理，并通过Illumina下一代测序技术进行测序。获得了两种新型细小病毒（EhPV2和EhPV3）的完整基因组以及3个部分序列（BATPV61、BATPV890a和BATPV890b），分析表明，EhPV2和EhPV3的主要衣壳蛋白与PV1聚类，并且与PV1的核苷酸同一性为60-64%，因此代表了该属内细小病毒的新物种。其他细小病毒聚集在我们系统发育树的不同分支中，可能代表新的物种和/或属。这表明蝙蝠中细小病毒具有广泛的多样性，特别是在蝙蝠中，因此支持了当前的概念，即细小病毒的进化比单纯严格的细小病毒-宿主共同进化更为复杂。
