Wei Qiaoling, Zhang Ting, Fan Jiawen, Jiang Rui, Chang Qing, Hong Jin, Xu Gezhi
Ophthalmology Department, EYE & ENT Hospital of Fudan University, Shanghai 200031, China.
Key Laboratory of Visual Impairment and Restoration of Fudan University, Shanghai 200031, China.
Ann Transl Med. 2020 Mar;8(5):193. doi: 10.21037/atm.2020.01.63.
This study aimed to investigate differentially expressed proteins in the vitreous humor (VH) of pathological myopia (PM) and normal eyes.
VH samples were collected from patients undergoing surgical treatment for rhegmatogenous retinal detachment (RRD), idiopathic epiretinal membrane (ERM), myopic retinoschisis (MRS) or macular hole (MH). A label-free quantitative proteomic analysis was performed to detect the differentially expressed proteins, and expression of three differentially expressed proteins was confirmed by ELISA.
In PM patients (MH-PM, MRS-PM and RRD-PM), the expression of prostaglandin-H2 D-isomerase (PGDS) and glutathione peroxidase 3 (GPX3) was significantly lower than in controls (MH, ERM, and RRD). The versican core protein expression decreased in the PM group. The vitreous concentrations of PGDS and GPX3 in patients with axial length (AL) of 26.5-29.0 mm were higher than in patients with AL >29.0 mm or AL <26.5 mm. NRF-2 expression was the lowest in patients with AL >29.0 mm.
Our study provides new evidence on the molecular changes in the VH of PM patients, and these molecules have the potential to become new targets for the therapy of PM.
本研究旨在调查病理性近视（PM）患者与正常眼玻璃体液（VH）中差异表达的蛋白质。
从接受孔源性视网膜脱离（RRD）、特发性视网膜前膜（ERM）、近视性视网膜劈裂（MRS）或黄斑裂孔（MH）手术治疗的患者中收集VH样本。进行无标记定量蛋白质组学分析以检测差异表达的蛋白质，并通过酶联免疫吸附测定（ELISA）确认三种差异表达蛋白质的表达。
在PM患者（MH-PM、MRS-PM和RRD-PM）中，前列腺素-H2 D-异构酶（PGDS）和谷胱甘肽过氧化物酶3（GPX3）的表达明显低于对照组（MH、ERM和RRD）。多功能蛋白聚糖核心蛋白表达在PM组中降低。眼轴长度（AL）为26.5-29.0mm的患者玻璃体液中PGDS和GPX3的浓度高于AL>29.0mm或AL<26.5mm的患者。NRF-2表达在AL>29.0mm的患者中最低。
我们的研究为PM患者VH中的分子变化提供了新证据，这些分子有可能成为PM治疗的新靶点。
