Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan; Department of Dentistry, Cardinal Tien Hospital Yonghe Branch, New Taipei, Taiwan.
Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
Dent Mater. 2020 Jun;36(6):755-764. doi: 10.1016/j.dental.2020.03.018. Epub 2020 Apr 17.
In vital pulp therapy (VPT), a barrier is created with appropriate capping to protect the remaining pulp and thus maintain pulp vitality. Here, we evaluated the feasibility of a biphasic calcium phosphate cement (CPC)-calcium sulfate hemihydrate (CSH) biomaterial containing simvastatin (Sim) and collagenase (Col) for VPT.
Combinations of varying CPC and CSH concentrations were analyzed for their handling properties and setting times, with their structures observed through scanning electron microscopy-energy dispersive X-ray spectrometry (SEM-EDS). Drug release patterns of simvastatin and collagenase combined with CPC-CSH (CPC-CSH-Sim-Col) were also analyzed, followed by biocompatibility and bioactivity tests on human dental pulp stem cells (hDPSCs) and in vivo animal study in canine models; the in vivo results were obtained through microcomputed tomography and histological analysis.
The results revealed that 70 wt% CPC (CPC7) with 30 wt% CSH (CSH3) exhibited optimal setting time and porous structure for clinical use. The cell viability and cytotoxicity analysis demonstrated that CPC7-CSH3 with or without simvastatin or collagenase did not injure hDPSCs. In vivo, the CPC7-CSH3-Sim-Col induced dentin bridge formation.
CPC7-CSH3-Sim-Col in this study has great potential as a VPT biomaterial to enhance the dentin bridge formation.
在活髓治疗（VPT）中，通过适当的盖髓来创建屏障，以保护剩余的牙髓，从而维持牙髓活力。在此，我们评估了含有辛伐他汀（Sim）和胶原酶（Col）的双相磷酸钙水泥（CPC）-半水硫酸钙（CSH）生物材料用于 VPT 的可行性。
分析了不同 CPC 和 CSH 浓度组合的操作性能和凝固时间，并通过扫描电子显微镜-能谱仪（SEM-EDS）观察其结构。还分析了辛伐他汀和胶原酶与 CPC-CSH（CPC-CSH-Sim-Col）结合的药物释放模式，然后对人牙髓干细胞（hDPSCs）进行生物相容性和生物活性测试，并在犬模型中进行体内动物研究；通过微计算机断层扫描和组织学分析获得体内结果。
结果表明，70wt%的 CPC（CPC7）与 30wt%的 CSH（CSH3）具有最佳的凝固时间和适合临床应用的多孔结构。细胞活力和细胞毒性分析表明，含有或不含有辛伐他汀或胶原酶的 CPC7-CSH3 不会损伤 hDPSCs。在体内，CPC7-CSH3-Sim-Col 诱导了牙本质桥的形成。
本研究中的 CPC7-CSH3-Sim-Col 作为一种 VPT 生物材料具有很大的潜力，可以增强牙本质桥的形成。
