Woodward B C
J Clin Microbiol. 1982 Aug;16(2):367-72. doi: 10.1128/jcm.16.2.367-372.1982.
An evaluation of the enzyme-linked immunosorbent assay (ELISA) as a screening test for immunoglobulin G class-specific anti-Toxoplasma antibodies was conducted. Results for 191 serum specimens assayed by the ELISA, indirect hemagglutination (IHA), and methylene blue dye tests indicated that good qualitative agreement exists among these tests. High ELISA titers relative to methylene blue dye titers may be attributed in part to the presence of rheumatoid factor. ELISA results were found to be reproducible. A parallel study of 1,760 sera assayed by ELISA and IHA was performed with 3.0-mm-diameter serum filter paper spots as a source of specimen. When a 1:64 titer and 40 IU were used as threshold values for IHA and the ELISA, respectively, 4.8% of the specimens tested were positive in both tests. An additional 2% of these specimens were ELISA positive and IHA negative, whereas 2% were ELISA negative and IHA positive. The total number of positive specimens by either test (7%) closely agreed with previously reported results. In view of these data, the simplicity of the test, and relative economic considerations, the ELISA may be considered an alternative method for screening for anti-Toxoplasma antibodies.
对酶联免疫吸附测定（ELISA）作为免疫球蛋白G类特异性抗弓形虫抗体筛查试验进行了评估。通过ELISA、间接血凝试验（IHA）和亚甲蓝染色试验检测的191份血清标本结果表明，这些试验之间存在良好的定性一致性。相对于亚甲蓝染色滴度而言，ELISA高滴度可能部分归因于类风湿因子的存在。发现ELISA结果具有可重复性。以直径3.0毫米的血清滤纸片斑点作为标本来源，对1760份血清进行了ELISA和IHA平行研究。当分别将1:64滴度和40 IU用作IHA和ELISA的阈值时，4.8%的检测标本在两种试验中均为阳性。这些标本中另外2%为ELISA阳性而IHA阴性，2%为ELISA阴性而IHA阳性。两种试验中任何一种试验的阳性标本总数（7%）与先前报告的结果非常一致。鉴于这些数据、试验的简便性以及相对经济方面的考虑，ELISA可被视为筛查抗弓形虫抗体的替代方法。
